Global Patent Index - EP 1409719 A1

EP 1409719 A1 20040421 - METHOD FOR DETECTING KNOWN MUTATIONS IN TUBE

Title (en)

METHOD FOR DETECTING KNOWN MUTATIONS IN TUBE

Title (de)

VERFAHREN ZUM NACHWEIS VON GEKANTEN POLYMORPHISMEN

Title (fr)

PROCEDE DE DETECTION DE MUTATIONS CONNUES EN TUBE

Publication

EP 1409719 A1 20040421 (FR)

Application

EP 01963075 A 20010808

Priority

  • FR 0102574 W 20010808
  • FR 0010425 A 20000808

Abstract (en)

[origin: WO0212557A1] The invention concerns a method for detecting a mutation in a target nucleic acid comprising amplification of the target DNA, specific hybridization of a probe with the DNA target, extension of the probe by selective addition of alpha S-phosphothioatedesoxynucleotide complementary of the mutation in a tube A and adding an alpha S-phosphothioatedesoxynucleotide complementary of the natural base corresponding to said mutation in a tube B; the resulting extended probe being resistant to digestion by an exonuclease, particularly by exonuclease III.

IPC 1-7

C12Q 1/68

IPC 8 full level

C12Q 1/68 (2006.01); C12Q 1/6827 (2018.01)

CPC (source: EP US)

C12Q 1/6827 (2013.01 - EP US)

C-Set (source: EP US)

C12Q 1/6827 + C12Q 2565/537 + C12Q 2525/125 + C12Q 2521/307

Citation (search report)

See references of WO 0212557A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

DOCDB simple family (publication)

WO 0212557 A1 20020214; AU 8412101 A 20020218; EP 1409719 A1 20040421; FR 2812885 A1 20020215; US 2004076969 A1 20040422

DOCDB simple family (application)

FR 0102574 W 20010808; AU 8412101 A 20010808; EP 01963075 A 20010808; FR 0010425 A 20000808; US 34409303 A 20030808