EP 1409742 A2 20040421 - TEST-KIT FOR DETECTING ENTEROHEMORRHAGIC ESCHERICHIA COLI STRAINS (EHEC)
Title (en)
TEST-KIT FOR DETECTING ENTEROHEMORRHAGIC ESCHERICHIA COLI STRAINS (EHEC)
Title (de)
TEST-KIT ZUM NACHWEIS VON ENTEROHÄMORRHAGISCHEN ESCHERICHIA COLI-STÄMMEN (EHEC)
Title (fr)
KIT DE TEST POUR DETERMINER LA PRESENCE DE SOUCHES ESCHERICHIA COLI ENTEROHEMORRHAGIQUES (EHEC)
Publication
Application
Priority
- AT 0200222 W 20020726
- AT 11722001 A 20010726
Abstract (en)
[origin: WO03010332A2] The invention relates to a test-kit for detecting enterohemorrhagic escherichia coli strains (EHEC), containing, each time, a primer pair for amplifying at least a portion of the SLT1 gene, SLT2 gene, r DOLLAR m(IbE) gene, eaeA gene and of a control gene in a single reaction and containing an oligonucleotide serving as a trapping probe and an oligonucleotide serving as a detector probe for hybridizing, at room temperature, the amplification products that are obtained with the primers. According to the invention, all trapping probes are immobilized on a common solid surface, and the detector probes are provided with a visually detectable marking. The use of a test kit enables a simple and reliable detection of enterohemorrhagic escherichia coli strains in a single reaction.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01); C12Q 1/689 (2018.01)
CPC (source: EP)
C12Q 1/689 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01)
Citation (search report)
See references of WO 03010332A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR
DOCDB simple family (publication)
WO 03010332 A2 20030206; WO 03010332 A3 20031106; AT 411832 B 20040625; AT A11722001 A 20031115; EP 1409742 A2 20040421
DOCDB simple family (application)
AT 0200222 W 20020726; AT 11722001 A 20010726; EP 02762153 A 20020726