EP 1417317 A2 20040512 - METHOD FOR PRODUCING A MARKER-FREE MUTATED TARGET ORGANISM AND PLASMID VECTORS SUITABLE FOR THE SAME
Title (en)
METHOD FOR PRODUCING A MARKER-FREE MUTATED TARGET ORGANISM AND PLASMID VECTORS SUITABLE FOR THE SAME
Title (de)
VERFAHREN ZUR HERSTELLUNG EINES MARKER-FREIEN MUTIERTEN ZIELORGANISMUS SOWIE DAFÜR GEEIGNETE PLASMIDVEKTOREN
Title (fr)
PROCEDE DE PRODUCTION D'UN ORGANISME CIBLE MUTANT DEPOURVU DE GENE MARQUEUR ET VECTEUR PLASMIDIQUE UTILISE A CET EFFET
Publication
Application
Priority
- DE 10137815 A 20010806
- EP 0208231 W 20020724
Abstract (en)
[origin: WO03014362A2] The invention relates to a plasmid vector which does not replicate in a target organism and contains the following constituents: a) an origin of replication for a host organism which is not identical to the target organism, b) at least one genetic marker, c) optionally a section of a sequence which enables the transfer of DNA by means of conjugation (mob sequence), d) a section of a sequence which is homologous to sequences of the target organism and enables homologous recombination in the target organism, e) a gene for a galactokinase under the control of a promoter.
IPC 1-7
IPC 8 full level
C12N 1/21 (2006.01); C12N 15/09 (2006.01); C12N 15/54 (2006.01); C12N 15/77 (2006.01)
CPC (source: EP KR US)
C12N 15/63 (2013.01 - KR); C12N 15/64 (2013.01 - KR); C12N 15/77 (2013.01 - EP KR US)
Citation (search report)
See references of WO 03014362A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR
DOCDB simple family (publication)
WO 03014362 A2 20030220; WO 03014362 A3 20030501; CA 2456222 A1 20030220; DE 10137815 A1 20030227; EP 1417317 A2 20040512; JP 2004538003 A 20041224; KR 20040020080 A 20040306; US 2004171160 A1 20040902
DOCDB simple family (application)
EP 0208231 W 20020724; CA 2456222 A 20020724; DE 10137815 A 20010806; EP 02794516 A 20020724; JP 2003519491 A 20020724; KR 20047001776 A 20020724; US 48612504 A 20040206