EP 1421173 A2 20040526 - METHOD AND DEVICE FOR THE IN VITRO CULTIVATION OF CELLS
Title (en)
METHOD AND DEVICE FOR THE IN VITRO CULTIVATION OF CELLS
Title (de)
VERFAHREN UND VORRICHTUNG ZUR IN VITRO VERMEHRUNG VON ZELLEN
Title (fr)
PROCEDE ET DISPOSITIF DE CULTURE CELLULAIRE IN VITRO
Publication
Application
Priority
- CH 0200471 W 20020829
- CH 16192001 A 20010830
Abstract (en)
[origin: WO03020871A2] The invention relates to a method for the in vitro cultivation of cells, which grow on a culture surface. According to the invention, the cells are sown on culture surfaces (1) and are cultivated in a culture medium, whereby the culture surface is continually or periodically expanded, without being removed from the culture medium. To expand the culture surface, the cells are not detached from the culture surface and the culture surface between the cells is expanded. However, at least part of the cells can be detached and the culture surface can be expanded by the flooding of additional culture surface areas. The culture surface of an example device for said cell cultivation consists of one side of an expanding membrane (6), which is expanded by modifying the pressure on the other side. Said cell cultivation can be carried out without manual passaging. The cells are subjected to less stress than in known cell cultivation methods and said method can be automated more easily.
IPC 1-7
IPC 8 full level
C12M 1/00 (2006.01); C12M 3/00 (2006.01); C12M 3/02 (2006.01); C12M 3/04 (2006.01); C12N 5/02 (2006.01); C12N 5/06 (2006.01); C12N 5/07 (2010.01); C12N 5/071 (2010.01); C12N 5/08 (2006.01)
CPC (source: EP US)
C12M 25/02 (2013.01 - EP US); C12M 33/00 (2013.01 - EP US)
Citation (search report)
See references of WO 03020871A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR
DOCDB simple family (publication)
WO 03020871 A2 20030313; WO 03020871 A3 20030710; CA 2458941 A1 20030313; EP 1421173 A2 20040526; JP 2005500860 A 20050113; US 2004219668 A1 20041104
DOCDB simple family (application)
CH 0200471 W 20020829; CA 2458941 A 20020829; EP 02758026 A 20020829; JP 2003525575 A 20020829; US 48801804 A 20040227