Global Patent Index - EP 1427852 A4

EP 1427852 A4 20051228 - SINGLE NUCLEOTIDE AMPLIFICATION AND DETECTION BY POLYMERASE

Title (en)

SINGLE NUCLEOTIDE AMPLIFICATION AND DETECTION BY POLYMERASE

Title (de)

EINZELNUKLEOTIDAMPLIFIKATION UND -NACHWEIS MIT POLYMERASE

Title (fr)

DETECTION ET AMPLIFICATION D'UN NUCLEOTIDE PAR POLYMERASE

Publication

EP 1427852 A4 20051228 (EN)

Application

EP 02759494 A 20020829

Priority

  • US 0227564 W 20020829
  • US 31579801 P 20010829
  • US 11302502 A 20020401

Abstract (en)

[origin: WO03020891A2] A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, a non-hydrolyzable primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and an enzyme having 3'->5' exonuclease activity which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species characteristic of the sample; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on the detection.

IPC 1-7

C12Q 1/68; C12P 19/34; C07H 21/02; C07H 21/04; C07H 19/00

IPC 8 full level

G01N 33/53 (2006.01); C07H 19/10 (2006.01); C07H 19/20 (2006.01); C07H 21/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/42 (2006.01); C12Q 1/48 (2006.01); C12Q 1/68 (2006.01); G01N 33/566 (2006.01)

CPC (source: EP US)

C12Q 1/6823 (2013.01 - EP US)

Citation (search report)

  • [XY] WO 9414972 A1 19940707 - HYMAN EDWARD DAVID [US]
  • [Y] WO 0127153 A1 20010419 - SMITHKLINE BEECHAM CORP [US], et al
  • [Y] WO 9423055 A1 19941013 - US BIOCHEMICAL CORP [US]
  • [PXPY] WO 0240126 A2 20020523 - KECK GRADUATE INST [US], et al
  • [E] WO 03020984 A2 20030313 - AMERSHAM BIOSCIENCES CORP [US]
  • [E] WO 03020734 A2 20030313 - AMERSHAM BIOSCIENCES CORP [US]
  • [XY] ARZUMANOV A A ET AL: "Gamma-Phosphate-substituted 2'-deoxynucleoside 5'-triphosphates as substrates for DNA polymerases", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 271, no. 40, 4 October 1996 (1996-10-04), pages 24389 - 24394, XP002256215, ISSN: 0021-9258
  • [XY] VASSILIOU W ET AL: "Exploiting Polymerase Promiscuity: A Simple Colorimetric RNA Polymerase Assay", VIROLOGY, ACADEMIC PRESS,ORLANDO, US, vol. 274, no. 2, 1 September 2000 (2000-09-01), pages 429 - 437, XP004435943, ISSN: 0042-6822
  • [XY] NEWTON C R ET AL: "THE PRODUCTION OF PCR PRODUCTS WITH 5' SINGLE-STRANDED TAILS USING PRIMERS THAT INCORPORATE NOVEL PHOSPHORAMIDITE INTERMEDIATES", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 21, no. 5, 1993, pages 1155 - 1162, XP002041613, ISSN: 0305-1048
  • See references of WO 03020891A2

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR

DOCDB simple family (publication)

WO 03020891 A2 20030313; WO 03020891 A3 20030508; AT E458068 T1 20100315; AU 2002324826 B2 20071108; CA 2456906 A1 20030313; CA 2456906 C 20131210; DE 60235383 D1 20100401; DK 1427852 T3 20100607; EP 1427852 A2 20040616; EP 1427852 A4 20051228; EP 1427852 B1 20100217; JP 2005501551 A 20050120; JP 4360904 B2 20091111; US 2003096253 A1 20030522; US 7033762 B2 20060425

DOCDB simple family (application)

US 0227564 W 20020829; AT 02759494 T 20020829; AU 2002324826 A 20020829; CA 2456906 A 20020829; DE 60235383 T 20020829; DK 02759494 T 20020829; EP 02759494 A 20020829; JP 2003525595 A 20020829; US 11302502 A 20020401