EP 1430078 A2 20040623 - METHOD FOR INCREASING THE SOLUBILITY, EXPRESSION RATE AND THE ACTIVITY OF PROTEINS DURING RECOMBINANT PRODUCTION
Title (en)
METHOD FOR INCREASING THE SOLUBILITY, EXPRESSION RATE AND THE ACTIVITY OF PROTEINS DURING RECOMBINANT PRODUCTION
Title (de)
VERFAHREN ZUR ERHÖHUNG DER LÖSLICHKEIT, DER EXPRESSIONSRATE UND DER AKTIVITÄT VON PROTEINEN WÄHREND DER REKOMBINANTEN HERSTELLUNG
Title (fr)
PROCEDE POUR AUGMENTER LA SOLUBILITE, LE TAUX D'EXPRESSION ET L'ACTIVITE DE PROTEINES PENDANT LA PRODUCTION PAR RECOMBINAISON
Publication
Application
Priority
- DE 10145694 A 20010917
- EP 0210329 W 20020914
Abstract (en)
[origin: WO03025116A2] The invention relates to a method for producing a lysate containing auxiliary proteins. According to said method, a strain, which is suitable for obtaining in vitro translation lysates is transformed using a vector containing one or more genes that code for one or more auxiliary proteins, the auxiliary proteins being expressed in said strain and the lysate containing auxiliary proteins being obtained from said strains. The invention also relates to a lysate containing auxiliary proteins that can be obtained according to the inventive method, to blends of said lysates and to the use of the lysates and blends in in vitro translation systems.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C07K 14/00 (2006.01); C07K 14/245 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 9/12 (2006.01); C12N 15/67 (2006.01); C12P 1/04 (2006.01); C12P 21/02 (2006.01)
CPC (source: EP US)
C12N 9/1241 (2013.01 - EP US); C12N 15/67 (2013.01 - EP US)
Citation (search report)
See references of WO 03025116A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR
DOCDB simple family (publication)
WO 03025116 A2 20030327; WO 03025116 A3 20040311; DE 10145694 A1 20030403; EP 1430078 A2 20040623; JP 2005503157 A 20050203; US 2004248238 A1 20041209
DOCDB simple family (application)
EP 0210329 W 20020914; DE 10145694 A 20010917; EP 02774617 A 20020914; JP 2003529890 A 20020914; US 48994104 A 20040317