EP 1432817 A1 20040630 - METHODS AND ARRAYS FOR DETECTING BIOLOGICAL MOLECULES
Title (en)
METHODS AND ARRAYS FOR DETECTING BIOLOGICAL MOLECULES
Title (de)
VERFAHREN UND ARRAYS ZUM NACHWEIS BIOLOGISCHER MOLEKÜLE
Title (fr)
PROCEDES ET RESEAUX DE DETECTION DE MOLECULES BIOLOGIQUES
Publication
Application
Priority
- US 0219346 W 20020618
- US 32931101 P 20011016
Abstract (en)
[origin: WO03029490A1] Arrays and methods for detecting one or more biological molecules, where the methods generally comprise the steps of: providing a first support immobilized with one or more reagents; providing a second support immobilized with one on more of ligands; contacting the reagents immobilized to the first support with the ligands immobilized on the second support whereby one or more of the reagents bind to one or more of the ligands; and separating the first support from the second support so that one or more of the bound reagents remain bound to one or more ligands on the second support after separation. In one preferred method, proteins are immobilized on a support with adequate strength so that the proteins can be dissociated from the support under certain conditions, such as after binding with other proteins immobilized on another support. For example, antibody arrays produced according to the present invention may be used to detect protein expressions in a protein lysate and may be used in immunostaining to reveal the presence and location of proteins in cells.
IPC 1-7
C12Q 1/68; C12Q 1/70; C07H 21/04; C07H 21/00; G01N 33/53; C07K 16/00
IPC 8 full level
C12Q 1/68 (2006.01); B01J 19/00 (2006.01); G01N 33/50 (2006.01); G01N 33/543 (2006.01); G01N 33/68 (2006.01); C07B 61/00 (2006.01)
CPC (source: EP)
B01J 19/0046 (2013.01); B82Y 30/00 (2013.01); C12Q 1/6837 (2013.01); G01N 33/5005 (2013.01); G01N 33/54306 (2013.01); G01N 33/54366 (2013.01); G01N 33/6842 (2013.01); G01N 33/6854 (2013.01); B01J 2219/00387 (2013.01); B01J 2219/00497 (2013.01); B01J 2219/0052 (2013.01); B01J 2219/00527 (2013.01); B01J 2219/00576 (2013.01); B01J 2219/00659 (2013.01); B01J 2219/00673 (2013.01); B01J 2219/00675 (2013.01); B01J 2219/00677 (2013.01); B01J 2219/00722 (2013.01); B01J 2219/00725 (2013.01); B01J 2219/00743 (2013.01); C07B 2200/11 (2013.01); C40B 40/00 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 03029490 A1 20030410; EP 1432817 A1 20040630; EP 1432817 A4 20061227
DOCDB simple family (application)
US 0219346 W 20020618; EP 02742197 A 20020618