EP 1436391 A2 20040714 - LOW-TEMPERATURE CYCLE EXTENSION OF DNA WITH HIGH PRIMING SPECIFICITY
Title (en)
LOW-TEMPERATURE CYCLE EXTENSION OF DNA WITH HIGH PRIMING SPECIFICITY
Title (de)
DNA-VERLÄNGERUNG IM NIEDRIGTEMPERATURZYKLUS MIT HOHER PRIMING-SPEZIFITÄT
Title (fr)
EXTENSION CYCLIQUE A BASSE TEMPERATURE D'ADN A HAUTE SPECIFICITE D'AMORCAGE
Publication
Application
Priority
- IB 0203341 W 20020605
- US 87813101 A 20010608
Abstract (en)
[origin: WO02101004A2] The invention relates to methods for extending a primer or a pair of primers in low-temperature cycle DNA amplification for cycle sequencing and PCR. In particular, the methods contemplate the combined use of moderately thermostable DNA polymerases in the presence of a low concentration of glycerol or ethylene glycol, or the mixtures thereof, as an agent to reduce the melting temperature of DNA (that is, the temperature at which the double-strands of DNA are denatured). Predistributed reaction mixtures of a high-fidelity and high processivity DNA polymerase stable at room temperature for several weeks in ready-to-use kits are also contemplated by the invention.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/686 (2013.01); C12Q 2535/113 (2013.01)
C-Set (source: EP)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 02101004 A2 20021219; WO 02101004 A3 20040429; CA 2449560 A1 20021219; EP 1436391 A2 20040714; EP 1436391 A4 20050216; JP 2005514003 A 20050519; RU 2004100107 A 20050610
DOCDB simple family (application)
IB 0203341 W 20020605; CA 2449560 A 20020605; EP 02758704 A 20020605; JP 2003503756 A 20020605; RU 2004100107 A 20020605