EP 1436626 A2 20040714 - METHOD FOR SELECTING HLA-DP4 LIGANDS
Title (en)
METHOD FOR SELECTING HLA-DP4 LIGANDS
Title (de)
VERFAHREN ZUR SELEKTION VON HLA-DP4-LIGANDEN
Title (fr)
PROCEDE DE SELECTION DE LIGANDS D'HLA-DP4
Publication
Application
Priority
- FR 0203555 W 20021017
- FR 0113352 A 20011017
Abstract (en)
[origin: FR2830940A1] Process for selecting ligands (A) of HLA (human leukocyte antigen)-DP4, is new. Process for selecting ligands (A) of HLA (human leukocyte antigen)-DP4 comprising: (a) incubating purified DP4 with a labeled peptide (I) in presence of different concentrations of test compounds; (b) separating complexes formed; (c) determining DP4-(I) complexes by measuring a signal from the label; and (d) selecting compounds having binding IC50 less than 1000 nM, corresponding to the concentration required to inhibit 50 % binding of (I). (I) has signal-to-noise ratio over 5, at 10 nM, in a direct binding test to DP4. Independent claims are also included for the following; (1) (A), natural or synthetic, organic or inorganic, identified by the novel process; (2) nucleic acid (II) that encodes (A) when these are peptides; (3) expression cassette (EC) containing at least one (II) and regulatory sequences; (4) vector containing (II) or EC; (5) cell transformed with at least one (II) or vector of (4); (6) diagnosing the immune state of an individual by in vitro contact between test sample and (A), then detecting CD4+ T cells specific for an antigen; (7) kit comprising at least one (A) and system for detecting antigen-specific CD4+ T cells; and (8) selecting antigen-specific CD4+ T cells by contacting cells with a fluorescently labeled tetramer, prepared from complexes between (A) and soluble DP4, then recovering cells that bind to the tetramer by flow cytometry.
IPC 1-7
IPC 8 full level
A61P 37/02 (2006.01); C07K 14/47 (2006.01); C12N 15/12 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP US)
A61P 37/02 (2017.12 - EP); C07K 14/47 (2013.01 - EP US); G01N 33/5008 (2013.01 - EP US); G01N 33/5047 (2013.01 - EP US); G01N 33/505 (2013.01 - EP US); G01N 33/5091 (2013.01 - EP US); G01N 33/68 (2013.01 - EP US)
Citation (search report)
See references of WO 03040299A2
Citation (examination)
- LEE ET AL, J. IMMUNOL., vol. 140, no. 4, 1988, pages 1063 - 1068
- SETTE ET AL, J. IMMUNOL., vol. 143, no. 4, 1989, pages 1265 - 1267
- DEPIL ET AL, VACCINE, vol. 24, 2006, pages 2225 - 2229
- GORDON ET AL, EUR. J. IMMUNOL., vol. 25, 1995, pages 1473 - 1476
- REICH ET AL, J. IMMUNOL., vol. 152, 1994, pages 2279 - 2288
- MUIXI ET AL, J. IMMUNOL., vol. 181, 2008, pages 795 - 807
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR
DOCDB simple family (publication)
FR 2830940 A1 20030418; FR 2830940 B1 20070615; AU 2002360128 A1 20030519; CA 2463705 A1 20030515; EP 1436626 A2 20040714; US 2005059107 A1 20050317; US 2010324829 A1 20101223; US 7718575 B2 20100518; WO 03040299 A2 20030515; WO 03040299 A3 20031127
DOCDB simple family (application)
FR 0113352 A 20011017; AU 2002360128 A 20021017; CA 2463705 A 20021017; EP 02795325 A 20021017; FR 0203555 W 20021017; US 49189104 A 20041014; US 71971210 A 20100308