EP 1438327 A1 20040721 - METHOD FOR RECONSTITUTING A RECOMBINANT PROTEIN TO ITS BIOLOGICALLY ACTIVE FORM
Title (en)
METHOD FOR RECONSTITUTING A RECOMBINANT PROTEIN TO ITS BIOLOGICALLY ACTIVE FORM
Title (de)
VERFAHREN ZUR WIEDERHERSTELLUNG EINES BIOLOGISCH-AKTIVEN REKOMBINANTEN PROTEINES
Title (fr)
PROCEDE DE RECONSTITUTION SOUS SA FORME ACTIVE D'UNE PROTEINE DE RECOMBINAISON
Publication
Application
Priority
- EP 02800587 A 20021001
- EP 0210992 W 20021001
- EP 01123698 A 20011003
Abstract (en)
[origin: EP1300415A1] In a method for reconstituting a recombinant protein from a denatured state to its active form, a feed solution containing the recombinant protein in its denatured and/or in biologically inactive intermediate forms is subjected to a chromatographic separation process, in which the protein is reconstituted under conditions that promote refolding of the protein and the intermediate forms are separated from the refolded protein. The denatured form and/or the inactive intermediate forms of the protein are separated from the refolded protein in a continuous or quasi-continuous manner and optionally recycled to the feed solution. <IMAGE>
IPC 1-7
C07K 1/113; C07K 1/16; C07K 1/18; C07K 1/20; C07K 1/22; C07K 1/36
IPC 8 full level
C07K 1/113 (2006.01); C07K 1/16 (2006.01); C07K 1/18 (2006.01); C07K 1/20 (2006.01); C07K 1/22 (2006.01); C07K 1/36 (2006.01)
CPC (source: EP KR)
C07K 1/1133 (2013.01 - EP); C07K 1/1136 (2013.01 - EP); C07K 1/16 (2013.01 - EP KR); C07K 1/36 (2013.01 - EP)
Citation (search report)
See references of WO 03031465A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR
DOCDB simple family (publication)
EP 1300415 A1 20030409; EP 1300415 B1 20070124; CA 2462232 A1 20030417; CA 2462232 C 20120103; DE 60126283 D1 20070315; DE 60126283 T2 20070705; EP 1438327 A1 20040721; EP 2298788 A1 20110323; JP 2005510481 A 20050421; JP 4264000 B2 20090513; KR 100930877 B1 20091210; KR 20040051594 A 20040618; WO 03031465 A1 20030417
DOCDB simple family (application)
EP 01123698 A 20011003; CA 2462232 A 20021001; DE 60126283 T 20011003; EP 0210992 W 20021001; EP 02800587 A 20021001; EP 10180934 A 20021001; JP 2003534447 A 20021001; KR 20047004379 A 20021001