EP 1438584 A1 20040721 - TARGET ASSISTED ITERATIVE SCREENING (TAIS) : A NOVEL SCREENING FORMAT FOR LARGE MOLECULAR REPERTOIRES
Title (en)
TARGET ASSISTED ITERATIVE SCREENING (TAIS) : A NOVEL SCREENING FORMAT FOR LARGE MOLECULAR REPERTOIRES
Title (de)
TARGET-ASSISTIERTES ITERATIVES SCREENING (TAIS): EIN NEUES SCREENING-FORMAT FÜR GROSSE MOLEKULARE REPERTOIRES
Title (fr)
CRIBLAGE ITERATIF ASSISTE PAR CIBLES (TAIS), NOUVEAU FORMAT DE CRIBLAGE POUR GRANDS REPERTOIRES MOLECULAIRES
Publication
Application
Priority
- US 0231349 W 20021001
- US 32656601 P 20011001
Abstract (en)
[origin: WO03029821A1] This invention provides a new in vitro screening method for the detection of protein-protein and other interactions. The method has been developed and applied to a commercial cDNA library to search for novel protein-protein interactions. PDZ, WW and SH3 domains from PSD95, Nedd4, Src, Abl and Crk proteins were used as test targets. 12 novel putative and 2 previously reported interactions were identified for 6 protein interaction modules in test screens. The novel screening format, dubbed TAIS (target-assisted iterative screening), provides an alternative platform to existing technologies for a pair-wise characterization of protein-protein, and other, interactions.
IPC 1-7
IPC 8 full level
C12N 15/10 (2006.01); C12P 21/04 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6883 (2018.01); C40B 30/04 (2006.01); C40B 40/02 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP)
C12N 15/1037 (2013.01); C12Q 1/6883 (2013.01); C40B 30/04 (2013.01); C40B 40/02 (2013.01); G01N 33/6845 (2013.01)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR
DOCDB simple family (publication)
WO 03029821 A1 20030410; CA 2462732 A1 20030410; EP 1438584 A1 20040721; EP 1438584 A4 20061227
DOCDB simple family (application)
US 0231349 W 20021001; CA 2462732 A 20021001; EP 02783996 A 20021001