EP 1440147 A2 20040728 - METHOD FOR ENHANCING KERATINOCYTE STEM CELLS
Title (en)
METHOD FOR ENHANCING KERATINOCYTE STEM CELLS
Title (de)
VERFAHREN ZUR ANREICHERUNG VON KERATINOZYTEN-STAMMZELLEN
Title (fr)
PROCEDE D'ENRICHISSEMENT DE CELLULES SOUCHES KERATINOCYTAIRES
Publication
Application
Priority
- FR 0203738 W 20021030
- FR 0114025 A 20011030
Abstract (en)
[origin: WO03038073A2] The invention concerns a method for enriching a population of keratinocyte stem cells, from a preparation of keratinocytes, comprising a pre-enrichment step in keratinocyte stem cells by adherence of said preparation on a constituent of extracellular matrices, in particular recognized by molecules involved in the adherence of the keratinocytes, in particular by the integrin family receptors, particularly collagen, for a period of time between about 5 and about 30 minutes, preferably between about 10 and about 20 minutes, advantageously 12 minutes, and in recuperating the stem cells which have adhered on said constituent of extracellular matrices by separation in conditions enabling to maintain the viabilty of the stem cells which have adhered, in particular by trypsinization, particularly using trypsin 0.05 % and EDTA 0.02 % to obtain a population of adherent cells.
IPC 1-7
IPC 8 full level
A61L 27/00 (2006.01); C12N 1/00 (2006.01); C12N 5/06 (2006.01); C12N 5/07 (2010.01); C12N 5/071 (2010.01); C12N 5/074 (2010.01); C12N 5/08 (2006.01); A61K 35/12 (2006.01)
CPC (source: EP US)
C12N 5/0629 (2013.01 - EP US); C12N 5/063 (2013.01 - EP US); A61K 35/12 (2013.01 - EP US)
Citation (search report)
See references of WO 03038073A2
Citation (examination)
JONES P H AND WATT F M: "Separation of human epidermal stem cells from transit amplifying cells on the basis of differences in integrin function and expression", CELL, vol. 73, 1993, pages 713 - 724, XP024245646
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR
DOCDB simple family (publication)
WO 03038073 A2 20030508; WO 03038073 A3 20031016; AU 2002352320 A1 20030512; CA 2464196 A1 20030508; EP 1440147 A2 20040728; FR 2831554 A1 20030502; FR 2831554 B1 20040820; JP 2005507258 A 20050317; US 2005106723 A1 20050519; US 7476538 B2 20090113
DOCDB simple family (application)
FR 0203738 W 20021030; AU 2002352320 A 20021030; CA 2464196 A 20021030; EP 02788031 A 20021030; FR 0114025 A 20011030; JP 2003540338 A 20021030; US 49427504 A 20041215