EP 1444524 A2 20040811 - METHODS FOR THE IDENTIFICATION OF MODULATORS OF MAGNESIUM CHELATASE EXPRESSION OR ACTIVITY IN PLANTS
Title (en)
METHODS FOR THE IDENTIFICATION OF MODULATORS OF MAGNESIUM CHELATASE EXPRESSION OR ACTIVITY IN PLANTS
Title (de)
VERFAHREN ZUR IDENTIFIZIERUNG VON MODULATOREN DER MAGNESIUM CHELATASE-AKTIVITÄT UND -EXPRESSION IN PFLANZEN
Title (fr)
PROCEDES POUR IDENTIFIER DES MODULATEURS DE L'EXPRESSION OU DE L'ACTIVITE DE LA CHELATASE DE MAGNESIUM DANS LES PLANTES
Publication
Application
Priority
US 0128761 W 20010914
Abstract (en)
[origin: WO0222857A2] The present inventors have discovered that Mg-chelatase is essential for the growth of <i>Arabidopsis</i>. Specifically, the inhibition of Mg-chelatase CHL,H gene expression in <i>Arabidopsis</i> seedlings results in varying levels of chlorosis (yellowing), significantly reduced growth and developmental abnormalities. Thus, <i>Arabidopsis</i> Mg-chelatase can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that modulate <i>Arabidopsis</i> Mg-chelatase expression or activity, comprising: contacting a compound with a <i>Arabidopsis</i> Mg-chelatase, or a subunit thereof, and detecting the presence and/or absence of binding between said compound and said Mg-chelatase, or detecting a change in Mg-chelatase expression or activity. The methods of the invention are useful for the identification of herbicides and other compounds that can modulate plant growth and development. In addition, the methods of the invention are useful for the identification of compounds that stimulate the expression or function of Mg-chelatase expression or function. Such compounds can be used to promote or manipulate plant growth and development.
[origin: WO0222857A2] The present inventors have discovered that Mg-chelatase is essential for the growth of Arabidopsis. Specifically, the inhibition of Mg-chelatase CHL,H gene expression in Arabidopsis seedlings results in varying levels of chlorosis (yellowing), significantly reduced growth and developmental abnormalities. Thus, Arabidopsis Mg-chelatase can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that modulate Arabidopsis Mg-chelatase expression or activity, comprising: contacting a compound with a Arabidopsis Mg-chelatase, or a subunit thereof, and detecting the presence and/or absence of binding between said compound and said Mg-chelatase, or detecting a change in Mg-chelatase expression or activity. The methods of the invention are useful for the identification of herbicides and other compounds that can modulate plant growth and development. In addition, the methods of the invention are useful for the identification of compounds that stimulate the expression or function of Mg-chelatase expression or function. Such compounds can be used to promote or manipulate plant growth and development.
IPC 1-7
IPC 8 full level
A01N 61/00 (2006.01); C12Q 1/25 (2006.01)
CPC (source: EP)
A01N 61/00 (2013.01); C12Q 1/25 (2013.01); G01N 2500/00 (2013.01)
Citation (search report)
See references of WO 0222857A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0222857 A2 20020321; WO 0222857 A3 20030619; WO 0222857 A8 20040617; EP 1444524 A2 20040811
DOCDB simple family (application)
US 0128761 W 20010914; EP 01970987 A 20010914