EP 1451346 A2 20040901 - METHOD OF LABELLING CRNAS FOR PROBING OLIGO-BASED MICROARRAYS
Title (en)
METHOD OF LABELLING CRNAS FOR PROBING OLIGO-BASED MICROARRAYS
Title (de)
VERFAHREN ZUR MARKIERUNG VON CRNAS ZUM ÜBERPRÜFEN AUF OLIGOS BASIERENDER MIKROARRAYS
Title (fr)
PROCEDE DE MARQUAGE D'ARNC POUR SONDER DES MICRORESEAUX D'OLIGODESOXYRIBONUCLEOTIDES
Publication
Application
Priority
- US 0226063 W 20020816
- US 31289301 P 20010816
Abstract (en)
[origin: WO03016483A2] A novel method of labelling cRNAs for probing oligo-based microarrays is disclosed. The polynucleotide template of interest is reverse transcribed into double-stranded cDNA using random primers that include an RNA polymerase promoter. This cDNA is then used as a template for synthesis of labeled RNA via in vitro transcription in the presence of labeled precursors.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6813 (2018.01); C12Q 1/6837 (2018.01); C12Q 1/6865 (2018.01)
CPC (source: EP)
C12Q 1/6813 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6865 (2013.01)
Citation (search report)
See references of WO 03016483A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR
DOCDB simple family (publication)
WO 03016483 A2 20030227; WO 03016483 A3 20040610; CA 2450215 A1 20030227; EP 1451346 A2 20040901; JP 2005502340 A 20050127
DOCDB simple family (application)
US 0226063 W 20020816; CA 2450215 A 20020816; EP 02757157 A 20020816; JP 2003521792 A 20020816