EP 1451366 A2 20040901 - MOLECULAR DETECTION SYSTEMS UTILIZING REITERATIVE OLIGONUCLEOTIDE SYNTHESIS
Title (en)
MOLECULAR DETECTION SYSTEMS UTILIZING REITERATIVE OLIGONUCLEOTIDE SYNTHESIS
Title (de)
REITERATIVE OLIGONUKLEOTIDSYNTHESE NUTZENDE MOLEKULARE NACHWEISSYSTEME
Title (fr)
SYSTEMES DE DETECTION MOLECULAIRE DANS LESQUELS ON UTILISE UNE SYNTHESE OLIGONUCLEOTIDIQUE REITERATIVE
Publication
Application
Priority
- US 0234419 W 20021029
- US 98466401 A 20011030
Abstract (en)
[origin: WO03038042A2] The present invention provides methods for detecting the presence of a target molecule by generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide synthesis events on a defined polynucleotide sequence. The methods generally comprise using a nucleoside, a mononucleotide, and oligonucleotide, or a polynucleotide, or analog thereof, to initiate synthesis of an oligonucleotide product that is substantially complementary to a target site on the defined polynucleotide sequence; optionally using nucleotides or nucleotide anologs as oligonucleotide chain elongators; using a chain terminator to terminate the polymerization reaction; and detecting multiple oligonucleotide products that have been synthesized by the polymerase. In one aspect, the invention provides a method for detecting a target protein, DNA or RNA by generating multiple detectable RNA oligoribonucleotides by abortive transcription.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/6858 (2013.01 - EP US); C12Q 2600/156 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR
DOCDB simple family (publication)
WO 03038042 A2 20030508; WO 03038042 A3 20040325; AT E460499 T1 20100315; AU 2002360306 A1 20030512; CA 2465158 A1 20030508; CA 2465158 C 20130122; DE 60235646 D1 20100422; EP 1451366 A2 20040901; EP 1451366 A4 20060913; EP 1451366 B1 20100310; JP 2006507792 A 20060309; US 2003099950 A1 20030529; US 2004137461 A1 20040715; US 2004157257 A1 20040812; US 2004175724 A1 20040909; US 2004234996 A1 20041125; US 2005026150 A1 20050203; US 2005064414 A1 20050324; US 7045319 B2 20060516; US 7226738 B2 20070605; US 7468261 B2 20081223; US 7470511 B2 20081230; US 7473775 B2 20090106; US 7541165 B2 20090602
DOCDB simple family (application)
US 0234419 W 20021029; AT 02795555 T 20021029; AU 2002360306 A 20021029; CA 2465158 A 20021029; DE 60235646 T 20021029; EP 02795555 A 20021029; JP 2003540307 A 20021029; US 48897104 A 20041018; US 60058103 A 20030623; US 60204503 A 20030624; US 60713603 A 20030627; US 68671303 A 20031017; US 79076604 A 20040303; US 98466401 A 20011030