Global Patent Index - EP 1451366 A4

EP 1451366 A4 20060913 - MOLECULAR DETECTION SYSTEMS UTILIZING REITERATIVE OLIGONUCLEOTIDE SYNTHESIS

Title (en)

MOLECULAR DETECTION SYSTEMS UTILIZING REITERATIVE OLIGONUCLEOTIDE SYNTHESIS

Title (de)

REITERATIVE OLIGONUKLEOTIDSYNTHESE NUTZENDE MOLEKULARE NACHWEISSYSTEME

Title (fr)

SYSTEMES DE DETECTION MOLECULAIRE DANS LESQUELS ON UTILISE UNE SYNTHESE OLIGONUCLEOTIDIQUE REITERATIVE

Publication

EP 1451366 A4 20060913 (EN)

Application

EP 02795555 A 20021029

Priority

  • US 0234419 W 20021029
  • US 98466401 A 20011030

Abstract (en)

[origin: US7541165B2] The present invention provides methods for detecting the presence of a target molecule by generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide synthesis events on a defined polynucleotide sequence. The methods generally comprise using a nucleoside, a mononucleotide, an oligonucleotide, or a polynucleotide, or analog thereof, to initiate synthesis of an oligonucleotide product that is substantially complementary to a target site on the defined polynucleotide sequence; optionally using nucleotides or nucleotide analogs as oligonucleotide chain elongators; using a chain terminator to terminate the polymerization reaction; and detecting multiple oligonucleotide products that have been synthesized by the polymerase. In one aspect, the invention provides a method for detecting a target protein, DNA or RNA by generating multiple detectable RNA oligoribonucleotides by abortive transcription.

IPC 1-7

C12Q 1/68; C12P 19/34; C12N 9/00; C07H 21/04

IPC 8 full level

C12Q 1/68 (2006.01)

CPC (source: EP US)

C12Q 1/6858 (2013.01 - EP US); C12Q 2600/156 (2013.01 - EP US)

C-Set (source: EP US)

  1. C12Q 1/6858 + C12Q 2535/101 + C12Q 2521/119
  2. C12Q 1/6858 + C12Q 2525/186 + C12Q 2525/143 + C12Q 2523/125 + C12Q 2521/119

Citation (search report)

  • [Y] EP 0369775 A2 19900523 - LIFECODES CORP [US]
  • [Y] US 5503979 A 19960402 - KRAMER FRED R [US], et al
  • [YD] DAUBE S S ET AL: "Functional transcription elongation complexes from synthetic RNA-DNA bubble duplexes", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 258, 20 November 1992 (1992-11-20), pages 1320 - 1324, XP002259448, ISSN: 0036-8075
  • [Y] DAUBE SHIRLEY S ET AL: "Coupling of RNA displacement and intrinsic termination in transcription from synthetic RNA cntdot DNA bubble duplex constructs", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 91, no. 20, 1994, pages 9539 - 9543, XP002390074, ISSN: 0027-8424
  • [Y] AIYAR S E ET AL: "A mismatch bubble in double-stranded DNA suffices to direct precise transcription initiation by Escherichia coli RNA polymerase.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. 6 MAY 1994, vol. 269, no. 18, 6 May 1994 (1994-05-06), pages 13179 - 13184, XP002390075, ISSN: 0021-9258
  • [Y] HANNA M M ET AL: "Topography of transcription: path of the leading end of nascent RNA through the Escherichia coli transcription complex.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. JUL 1983, vol. 80, no. 14, July 1983 (1983-07-01), pages 4238 - 4242, XP002390076, ISSN: 0027-8424
  • [Y] SASAKI N ET AL: "Transcriptional sequencing: A method for DNA sequencing using RNA polymerase.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 31 MAR 1998, vol. 95, no. 7, 31 March 1998 (1998-03-31), pages 3455 - 3460, XP002390077, ISSN: 0027-8424
  • [Y] KINSELLA L ET AL: "RNA polymerase: correlation between transcript length, abortive product synthesis, and formation of a stable ternary complex.", BIOCHEMISTRY. 25 MAY 1982, vol. 21, no. 11, 25 May 1982 (1982-05-25), pages 2719 - 2723, XP002390078, ISSN: 0006-2960
  • [Y] HANNA M M ET AL: "Probing the environment of nascent RNA in Escherichia coli transcription elongation complexes utilizing a new fluorescent ribonucleotide analog.", NUCLEIC ACIDS RESEARCH. 1 MAR 1999, vol. 27, no. 5, 1 March 1999 (1999-03-01), pages 1369 - 1376, XP002390079, ISSN: 0305-1048
  • [Y] GRIEP M A ET AL: "FLUORESCENCE ENERGY TRANSFER BETWEEN THE PRIMER AND THE BETA SUBUNIT OF THE DNA POLYMERASE III HOLOENZYME", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 267, no. 5, 15 February 1992 (1992-02-15), pages 3052 - 3059, XP001097562, ISSN: 0021-9258
  • [YD] PROMMER M ET AL: "A GENOMIC SEQUENCING PROTOCOL THAT YIELDS A POSITIVE DISPLAY OF 5-METHYLCYTOSINE RESIDUES IN INDIVIDUAL DNA STRANDS", FROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 89, March 1992 (1992-03-01), pages 1827 - 1831, XP009016394, ISSN: 0027-8424
  • [Y] MANCINI DEBORA ET AL: "Constitutively methylated CpG dinucleotides as mutation hot spots in the retinoblastoma gene (RB1)", AMERICAN JOURNAL OF HUMAN GENETICS, vol. 61, no. 1, 1997, pages 80 - 87, XP002390080, ISSN: 0002-9297
  • [Y] COSTAS C ET AL: "RNA-protein crosslinking to AMP residues at internal positions in RNA with a new photocrosslinking ATP analog.", NUCLEIC ACIDS RESEARCH. 1 MAY 2000, vol. 28, no. 9, 1 May 2000 (2000-05-01), pages 1849 - 1858, XP002390081, ISSN: 1362-4962
  • [A] PICKETTS D J ET AL: "DIFFERENTIAL TERMINATION OF PRIMER EXTENSION: A NOVEL, QUANTIFIABLE METHOD FOR DETECTION OF POINT MUTATIONS", HUMAN GENETICS, BERLIN, DE, vol. 89, 1992, pages 155 - 157, XP002910526, ISSN: 0340-6717

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR

DOCDB simple family (publication)

WO 03038042 A2 20030508; WO 03038042 A3 20040325; AT E460499 T1 20100315; AU 2002360306 A1 20030512; CA 2465158 A1 20030508; CA 2465158 C 20130122; DE 60235646 D1 20100422; EP 1451366 A2 20040901; EP 1451366 A4 20060913; EP 1451366 B1 20100310; JP 2006507792 A 20060309; US 2003099950 A1 20030529; US 2004137461 A1 20040715; US 2004157257 A1 20040812; US 2004175724 A1 20040909; US 2004234996 A1 20041125; US 2005026150 A1 20050203; US 2005064414 A1 20050324; US 7045319 B2 20060516; US 7226738 B2 20070605; US 7468261 B2 20081223; US 7470511 B2 20081230; US 7473775 B2 20090106; US 7541165 B2 20090602

DOCDB simple family (application)

US 0234419 W 20021029; AT 02795555 T 20021029; AU 2002360306 A 20021029; CA 2465158 A 20021029; DE 60235646 T 20021029; EP 02795555 A 20021029; JP 2003540307 A 20021029; US 48897104 A 20041018; US 60058103 A 20030623; US 60204503 A 20030624; US 60713603 A 20030627; US 68671303 A 20031017; US 79076604 A 20040303; US 98466401 A 20011030