EP 1456657 A1 20040915 - TAGGED MICROPARTICLE COMPOSITIONS AND METHODS
Title (en)
TAGGED MICROPARTICLE COMPOSITIONS AND METHODS
Title (de)
MIKROPARTIKELZUSAMMENSETZUNGEN MIT TAG UND VERFAHREN
Title (fr)
COMPOSITIONS DE MICROPARTICULES MARQUEES ET PROCEDES ASSOCIES
Publication
Application
Priority
- US 0235907 W 20021108
- US 33776801 P 20011109
Abstract (en)
[origin: WO03042699A1] Compositions and methods are disclosed for detecting multiple target analytes in a sample using microparticles having molecular tags attached by cleavable linkages. Generally, an assay mixture is formed comprising a sample and a reagent comprising multiple such microparticles under conditions that permit stable complexes to form between binding moieties on the surfaces of the microparticles and the analytes. In one aspect of the invention, the a second binding composition is added so that complexes form among the microparticle-bound binding moieties, the analytes, and second binding moieties of the second binding composition. Such second binding moieties have cleavage-inducing moieties attached that upon activation cause the cleavage of the cleavable linkages and the release of molecular tags. Released molecular tags are separated and the presence and/or amount of the target analytes are determined based on the analysis of the released and separated molecular tags.
IPC 1-7
G01N 33/543; G01N 33/553; G01N 33/551; G01N 33/554; G01N 33/545; G01N 33/536; G01N 33/537; G01N 33/533; G01N 21/00; G01N 21/76; G01N 33/00; G01N 33/53
IPC 8 full level
C07B 61/00 (2006.01); C07D 405/12 (2006.01); C07D 493/10 (2006.01); C07D 495/04 (2006.01); G01N 27/447 (2006.01); G01N 30/88 (2006.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01)
CPC (source: EP)
C07D 405/12 (2013.01); C07D 493/10 (2013.01); C07D 495/04 (2013.01); G01N 33/54313 (2013.01); C40B 40/00 (2013.01)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR
DOCDB simple family (publication)
WO 03042699 A1 20030522; CA 2465594 A1 20030522; CA 2465594 C 20120207; EP 1456657 A1 20040915; EP 1456657 A4 20080312; JP 2005509859 A 20050414
DOCDB simple family (application)
US 0235907 W 20021108; CA 2465594 A 20021108; EP 02778794 A 20021108; JP 2003544481 A 20021108