Global Patent Index - EP 1463821 A4

EP 1463821 A4 20060531 - IN INCREASING GENE TRANSFER EFFICIENCY BY PRE-INCUBATION WITH ENDOTHELIAL CELLS

Title (en)

IN INCREASING GENE TRANSFER EFFICIENCY BY PRE-INCUBATION WITH ENDOTHELIAL CELLS

Title (de)

IN-ERHÖHTE GENTRANSFEREFFIZIENZ DURCH VORINKUBIERUNG MIT ENDOTHELZELLEN

Title (fr)

AUGMENTATION DE L'EFFICACITE DU TRANSFERT DE GENES PAR PRE-INCUBATION AVEC DES CELLULES ENDOTHELIALES

Publication

EP 1463821 A4 20060531 (EN)

Application

EP 02795952 A 20021220

Priority

  • US 0240719 W 20021220
  • US 34186801 P 20011221

Abstract (en)

[origin: WO03060138A1] The present invention relates to an in-vitro method of gene transfer into isolated hematopoietic progenitor stem cells, particularly CD34<+>38<->cells, and is accomplished by expanding the hematopoietic stem in a culture medium including endothelial cells, such as porcine brain microvascular endothelial cells or human brain vascular endothelial cells and may include at least one cytokine. The hematopoietic stem cells are then transduced using a suitable vector, such as Maloney Leukemia Virus vector. As a result of the described method, the gene transfer efficiency is greatly enhanced as compared to control methods. The described methods have potential clinical implications for use in gene therapy applications.

IPC 1-7

C12N 15/86; C12N 15/63; C12N 15/85; C12N 15/87; C12N 15/00; C12N 15/09; C12N 15/70; C12N 15/74; A01N 43/04; A61K 31/70

IPC 8 full level

C12N 15/09 (2006.01); C12N 5/10 (2006.01); C12N 15/87 (2006.01)

CPC (source: EP)

C12N 15/87 (2013.01); C12N 2799/027 (2013.01)

Citation (search report)

  • [X] CHUTE J P ET AL: "Preincubation with endothelial cell monolayers increases gene transfer efficiency into human bone marrow CD34+CD38-progenitor cells", HUMAN GENE THERAPY, vol. 11, 10 December 2000 (2000-12-10), pages 2515 - 2528, XP002963382, ISSN: 1043-0342
  • [X] CHUTE J P ET AL: "A COMPARATIVE STUDY OF THE CELL CYCLE STATUS AND PRIMITIVE CELL ADHESION MOLECULE PROFILE OF HUMAN CD34+CELLS CULTURED IN STROMA-FREE VERSUS PORCINE MICROVASCULAR ENDOTHELIAL CELL CULTURES", EXPERIMENTAL HEMATOLOGY, NEW YORK, NY, US, vol. 27, 1999, pages 370 - 379, XP000929912, ISSN: 0301-472X
  • [Y] CHU P ET AL: "RETROVIRUS-MEDIATED GENE TRANSFER INTO HUMAN HEMATOPOIETIC STEM CELLS", JOURNAL OF MOLECULAR MEDICINE, SPRINGER VERLAG, DE, vol. 76, 1998, pages 184 - 192, XP002939375, ISSN: 0946-2716
  • [Y] ROSLER E ET AL: "Cocultivation of umbilical cord blood cells with endothelial cells leads to extensive amplification of competent CD34+CD38-cells", EXPERIMENTAL HEMATOLOGY, NEW YORK, NY, US, vol. 28, 2000, pages 841 - 852, XP002963383, ISSN: 0301-472X
  • [A] ZIEGLER B L ET AL: "EXPANSION OF STEM AND PROGENITOR CELLS", CURRENT OPINION IN HEMATOLOGY, RAPID SCIENCE PUBLISHERS, PHILADELPHIA, PA, US, vol. 5, no. 6, November 1998 (1998-11-01), pages 434 - 440, XP001009708, ISSN: 1065-6251
  • See references of WO 03060138A1

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SI SK TR

DOCDB simple family (publication)

WO 03060138 A1 20030724; AU 2002360674 A1 20030730; EP 1463821 A1 20041006; EP 1463821 A4 20060531; JP 2005514071 A 20050519

DOCDB simple family (application)

US 0240719 W 20021220; AU 2002360674 A 20021220; EP 02795952 A 20021220; JP 2003560222 A 20021220