EP 1463841 A2 20041006 - METHOD FOR DETECTING CYTOSINE-METHYLATION PATTERNS BY EXPONENTIAL LIGATION OF HYBRIDISED PROBE OLIGO-NUCLEOTIDES (MLA)
Title (en)
METHOD FOR DETECTING CYTOSINE-METHYLATION PATTERNS BY EXPONENTIAL LIGATION OF HYBRIDISED PROBE OLIGO-NUCLEOTIDES (MLA)
Title (de)
VERFAHREN ZUM NACHWEIS VON CYTOSIN-METHYLIERUNGSMUSTERN DURCH EXPONENTIELLE LIGATION HYBRIDISIERTER SONDENOLIGONUKLEOTIDE (MLA)
Title (fr)
PROCEDE DE DETECTION DE MODELES DE METHYLATION DE LA CYTOSINE PAR LIGATION EXPONENTIELLE D'OLIGONUCLEOTIDES SONDES HYBRIDES (MLA)
Publication
Application
Priority
- DE 0300073 W 20030108
- DE 10201138 A 20020108
Abstract (en)
[origin: WO03057909A2] The invention relates to a method for detecting cytosine-methylation in DNA samples which consists of the following steps: firstly, a genomic DNA sample, which comprises background DNA and DNA which is to be examined, is chemically treated in such a way that all non-methylated cytosine bases are converted into uracil, whereby the 5-methyl cytosine bases remain unchanged. The DNA sample which has been chemically treated is then amplified by using at least 2 primer-oligo-nucleotides and a polymerase, the DNA which is to be examined with respect to the background DNA is preferred as a template. In the final step, the amplificates are analysed and on the basis of the presence of an amplificate and/or from the analysis of further positions information on the methylation status is deduced in the DNA which is to be examined.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01); C12Q 1/6858 (2018.01)
CPC (source: EP US)
C12Q 1/6858 (2013.01 - EP US)
Citation (search report)
See references of WO 03057909A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT SE SI SK TR
DOCDB simple family (publication)
WO 03057909 A2 20030717; WO 03057909 A3 20031016; AU 2003206620 A1 20030724; AU 2003206620 B2 20070809; DE 10201138 A1 20030717; DE 10201138 B4 20050310; EP 1463841 A2 20041006; JP 2005514035 A 20050519; US 2005064428 A1 20050324
DOCDB simple family (application)
DE 0300073 W 20030108; AU 2003206620 A 20030108; DE 10201138 A 20020108; EP 03704206 A 20030108; JP 2003558202 A 20030108; US 50104004 A 20040708