EP 1468114 A1 20041020 - LATE-PCR

Title (en)

LATE-PCR

Title (de)

LATE-PCR

Title (fr)

PCR TARDIF

Publication

EP 1468114 A1 20041020 (EN)

Application

EP 02795963 A 20021219

Priority

US 0240752 W 20021219
US 34188601 P 20011219
US 32089302 A 20021217

Abstract (en)

[origin: WO03054233A1] A non-symmetric polymerase chain reaction PCR amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25°C below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primerand-probe sets for performing the foregoing amplifications and assays.

[origin: WO03054233A1] A non−symmetric polymerase chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration−adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25°C below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low−temperature probe, or both. Kits for performing such assays and primer or primerand−probe sets for performing the foregoing amplifications and assays.

IPC 1-7

C12Q 1/68; C12P 19/34; C07H 19/00; C07H 21/02; C07H 21/04

IPC 8 full level

G01N 33/53 (2006.01); C07H 21/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 1/28 (2006.01); G01N 33/566 (2006.01)

CPC (source: EP US)

C07H 21/00 (2013.01 - EP US); C12Q 1/6806 (2013.01 - US); C12Q 1/6851 (2013.01 - EP US); C12Q 1/686 (2013.01 - EP US)

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SI SK TR

DOCDB simple family (publication)

WO 03054233 A1 20030703; AT E394508 T1 20080515; AU 2002360685 A1 20030709; CA 2511128 A1 20030703; CA 2511128 C 20110118; DE 60226500 D1 20080619; DK 1468114 T3 20080901; EP 1468114 A1 20041020; EP 1468114 A4 20050525; EP 1468114 B1 20080507; ES 2306807 T3 20081116; HK 1120088 A1 20090320; JP 2005512577 A 20050512; JP 4355211 B2 20091028; PT 1468114 E 20080819; US 2004053254 A1 20040318; US 2008280292 A1 20081113; US 2013210656 A1 20130815; US 7198897 B2 20070403; US 8367325 B2 20130205; US 9476092 B2 20161025

DOCDB simple family (application)

US 0240752 W 20021219; AT 02795963 T 20021219; AU 2002360685 A 20021219; CA 2511128 A 20021219; DE 60226500 T 20021219; DK 02795963 T 20021219; EP 02795963 A 20021219; ES 02795963 T 20021219; HK 09100199 A 20090108; JP 2003554933 A 20021219; PT 02795963 T 20021219; US 201313734684 A 20130104; US 32089302 A 20021217; US 70142807 A 20070202