EP 1468278 A1 20041020 - DEVICE, USE OF SAID DEVICE, AND SEPARATION AGENT FOR SEPARATING PARTICLES BY MEANS OF FREE FLOW ELECTROPHORESIS
Title (en)
DEVICE, USE OF SAID DEVICE, AND SEPARATION AGENT FOR SEPARATING PARTICLES BY MEANS OF FREE FLOW ELECTROPHORESIS
Title (de)
VORRICHTUNG, VERWENDUNG DER VORRICHTUNG UND TRENNMITTEL ZUM TRENNEN VON TEILCHEN IN DER FREE-FLOW-ELEKTROPHORESE
Title (fr)
DISPOSITIF, UTILISATION ASSOCIEE ET AGENT DE SEPARATION POUR SEPARER DES PARTICULES PAR ELECTROPHORESE LIBRE
Publication
Application
Priority
- CH 0300034 W 20030120
- CH 862002 A 20020121
- US 35236602 P 20020128
Abstract (en)
[origin: WO03060503A1] The invention relates to a free flow electrophoresis device, an associated method and separation agents for separating particles by means of one such device. Said FFE device comprises at least one separation chamber (14) through which a separation agent (8) can flow, said separation chamber being defined by a bottom, a cover, and a spacer for maintaining a distance between the same. The inventive FFE device comprises a dosing pump for transporting the separation agent (8) which is supplied to the separation chamber by means of admissions (15, 15') and leaves the same via outlets (16); electrodes (9, 10) for creating an electrical field in the separation agent (8); sample loading sites (17) for adding a mixture of particles (1, 1', 1'') to be separated; and fractioning sites (18) for removing the particles which are separated by means of FFE in the separation agent (8). Said FFE device comprises selectable charge carriers (4, 5) for combining with the particles to be separated (1', 1'') and for producing charge-modified particles (7', 7'') which - due to their selectively variable net surface charge - exhibit a different migration behaviour in the FFE device, to particles (7) which are not charge-modified. The inventive device is characterised in that it comprises guiding or focussing cushions (12, 13) arranged between the separation agent (8) and at least one electrode (9, 10). Said focussing cushions (12, 13) consist of a medium (20) which exhibits highly increased electrical conductivity in relation to the separation agent (8). Furthermore, said device comprises separate channels (15') for supplying said medium (20) to the focussing cushions (12, 13).
IPC 1-7
IPC 8 full level
G01N 27/447 (2006.01)
CPC (source: EP)
G01N 27/44726 (2013.01); G01N 27/44769 (2013.01); G01N 27/44795 (2013.01)
Citation (search report)
See references of WO 03060503A1
Citation (examination)
- VÖLKL A. ET AL: "Isolation of rat hepatic peroxisomes by means of immune free flow electrophoresis", ELECTROPHORESIS, vol. 18, 1997, pages 774 - 780, XP007901237
- BONDY B. ET AL: "Sodium chloride in separation medium enhances cell compatibility of free electrophoresis", ELECTROPHORESIS, vol. 16, 1995, pages 92 - 97, XP008008220
DOCDB simple family (publication)
WO 03060503 A1 20030724; AU 2003201252 A1 20030730; EP 1468278 A1 20041020
DOCDB simple family (application)
CH 0300034 W 20030120; AU 2003201252 A 20030120; EP 03729399 A 20030120