EP 1474529 B1 20081022 - SPECIFIC MULTIPLEX ANALYSIS OF NUCLEIC ACIDS
Title (en)
SPECIFIC MULTIPLEX ANALYSIS OF NUCLEIC ACIDS
Title (de)
SPEZIFISCHE MULTIPLEX-ANALYSE VON NUKLEINSÄUREN
Title (fr)
ANALYSE MULTIPLEX SPECIFIQUE D'ACIDES NUCLEIQUES
Publication
Application
Priority
- EP 03704579 A 20030210
- EP 0301276 W 20030210
- EP 02002854 A 20020208
- US 35451802 P 20020208
Abstract (en)
[origin: WO03066897A2] A method of detecting the presence or absence of variant nucleotides within target nucleic acid sequences, said method comprising the use of at least one pair of target-specific amplification primers capable of hybridizing to target nucleic acid sequences, and the use of at least one set of diagnostic primers, each set consisting of at least two types of diagnostic primers capable of hybridizing to corresponding target nucleic acid sequences 3´-relative to said amplification primers, such that each set of diagnostic primers is semi-nested relative to the corresponding pair of amplification primers. Additionally, the use of said method for genotyping, for diagnostic screening, for assaying the cytosine methylation status and for quantification of nucleic acids is disclosed.
IPC 8 full level
C12Q 1/68 (2006.01); C12P 19/34 (2006.01)
CPC (source: EP)
C12Q 1/6858 (2013.01)
Citation (examination)
- EP 0332435 A2 19890913 - ICI PLC [GB]
- NEWTON ET AL: "Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS).", NUCLEIC ACID RESEARCH, vol. 17, no. 7, - 1989, pages 2503 - 2516
- VON AHSEN ET AL, CLINICAL CHEMISTRY, vol. 45, no. 12, - 1999, pages 2094 - 2101
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT SE SI SK TR
DOCDB simple family (publication)
WO 03066897 A2 20030814; WO 03066897 A3 20040122; AT E412068 T1 20081115; AU 2003206869 A1 20030902; DK 1474529 T3 20090223; EP 1474529 A2 20041110; EP 1474529 B1 20081022
DOCDB simple family (application)
EP 0301276 W 20030210; AT 03704579 T 20030210; AU 2003206869 A 20030210; DK 03704579 T 20030210; EP 03704579 A 20030210