Global Patent Index - EP 1478746 A4

EP 1478746 A4 20050629 - HYALURONAN SYNTHASES AND METHODS OF MAKING AND USING SAME

Title (en)

HYALURONAN SYNTHASES AND METHODS OF MAKING AND USING SAME

Title (de)

HYALURONSYNTHASEN UND VERFAHREN ZUR HERSTELLUNG UND VERWENDUNG DAVON

Title (fr)

HYALURONAN SYNTHASES ET LEURS PROCEDES DE FABRICATION ET D'UTILISATION

Publication

EP 1478746 A4 20050629 (EN)

Application

EP 02794128 A 20021203

Priority

  • US 0238596 W 20021203
  • US 33610501 P 20011203
  • US 1177101 A 20011211

Abstract (en)

[origin: WO03048330A2] A functionally active hyaluronan synthase having at least one modified amino acid residue therein as compared to a corresponding functionally active native hyaluronan synthase such that the functionally active hyaluronan synthase has an altered enzymatic activity as compared to the corresponding functionally active native hyaluronan synthase is disclosed. Methods of producing hyaluronic acid utilizing a recombinant host cell having an expression construct encoding the functionally active hyaluronan synthase with altered enzymatic activity are also disclosed.

IPC 1-7

C12N 9/20; C12P 19/04

IPC 8 full level

C12N 9/10 (2006.01); C12P 19/26 (2006.01)

CPC (source: EP)

C12N 9/1051 (2013.01); C12P 19/26 (2013.01)

Citation (search report)

  • [X] HELDERMON COY ET AL: "Streptococcal hyaluronan synthases and the synthesis of "designer" hyaluronan", INTERNATIONAL CONGRESS SERIES; NEW FRONTIERS IN MEDICAL SCIENCES: REDEFINING HYALURONAN ELSEVIER SCIENCE B.V. {A}, SARA BURGERHARTSTRAAT 25, 1000 AE, AMSTERDAM, NETHERLANDS SERIES : INTERNATIONAL CONGRESS SERIES (ISSN 0531-5131), 2000, & SYMPOSIUM ON NEW FRONTIERS IN MEDICAL SCIENCES: REDEFINING HYALURONAN; PADUA, ITALY; JUNE 17-19, 1999, pages 41 - 50, XP009047179, ISSN: 0-444-50357-9
  • [X] JING WEI ET AL: "Dissection of the two transferase activities of the Pasteurella multocida hyaluronan synthase: Two active sites exist in one polypeptide", GLYCOBIOLOGY, vol. 10, no. 9, September 2000 (2000-09-01), pages 883 - 889, XP002326785, ISSN: 0959-6658
  • [X] YOSHIDA MAMORU ET AL: "In vitro synthesis of hyaluronan by a single protein derived from mouse HAS1 gene and characterization of amino acid residues essential for the activity", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 1, 7 January 2000 (2000-01-07), pages 497 - 506, XP002326786, ISSN: 0021-9258
  • [PX] KUMARI KSHAMA ET AL: "The streptococcal hyaluronan synthases are inhibited by sulfhydryl-modifying reagents, but conserved cysteine residues are not essential for enzyme function", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 16, 19 April 2002 (2002-04-19), pages 13943 - 13951, XP002326787, ISSN: 0021-9258
  • See references of WO 03048330A2

Citation (examination)

DEANGELIS P.: "Hyaluronan synthases: fascinating glycosyltransferases from vertebrates, bacterial pathogens, and algal viruses", CELLULAR AND MOLECULAR LIFE SCIENCES, vol. 56, no. 7-8, 15 November 1999 (1999-11-15), pages 670 - 682

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SI SK TR

DOCDB simple family (publication)

WO 03048330 A2 20030612; WO 03048330 A3 20040923; AU 2002359581 A1 20030617; AU 2002359581 B2 20080110; CA 2469566 A1 20030612; CN 1596307 A 20050316; EP 1478746 A2 20041124; EP 1478746 A4 20050629

DOCDB simple family (application)

US 0238596 W 20021203; AU 2002359581 A 20021203; CA 2469566 A 20021203; CN 02826694 A 20021203; EP 02794128 A 20021203