EP 1481258 A1 20041201 - HIGH-THROUGHPUT SCREENING METHOD FOR DETERMINING THE ENANTIOSELECTIVITY OF CATALYSTS, BIOCATALYSTS, AND AGENTS
Title (en)
HIGH-THROUGHPUT SCREENING METHOD FOR DETERMINING THE ENANTIOSELECTIVITY OF CATALYSTS, BIOCATALYSTS, AND AGENTS
Title (de)
EIN HOCH-DURCHSATZ-SCREENING-VERFAHREN ZUR BESTIMMUNG DER ENANTIOSELEKTIVIT T VON KATALYSATOREN, BIOK ATALYSATOREN UND AGENZIEN
Title (fr)
PROCEDE DE SELECTION A HAUT RENDEMENT POUR LA DETERMINATION DE L'ENANTIOSELECTIVITE DE CATALYSEURS, DE BIOCATALYSEURS ET D'AGENTS
Publication
Application
Priority
- DE 10209177 A 20020301
- EP 0301825 W 20030222
Abstract (en)
[origin: WO03075031A1] The invention relates to a high-throughput screening method based on NMR spectroscopy for determining the enantioselectivity of reactions which show an asymmetric course. The reactions can be caused by chiral catalysts, agents, or biocatalysts such that said products can be evaluated regarding the enantioselectivity thereof. In one embodiment, isotope-marked pseudo-enantiomers or pseudo-prochiral substrates are used such that the enantioselectivity can be quantified by integrating the NMR signals of the respective substrates and/or products. The use of an automated setup of devices, including microtiter plates, robots, and high-throughput NMR devices, is decisive for the high-throughput process. In a second embodiment of the invention, the automated setup of devices is used to detect in a quantitative manner the products and/or educts that have been derivatized with enantiomer-pure agents in the form of diastereomers. At least 1000 ee determinations can be done per day with an accuracy of at least +/- 5 percent in both embodiments.
IPC 1-7
IPC 8 full level
G01N 24/08 (2006.01); C07C 27/02 (2006.01); C07C 31/12 (2006.01); C07C 33/20 (2006.01); C07C 69/013 (2006.01); C07C 69/145 (2006.01); G01N 37/00 (2006.01); G01R 33/30 (2006.01); G01R 33/46 (2006.01)
CPC (source: EP US)
G01R 33/46 (2013.01 - EP US)
Citation (search report)
See references of WO 03075031A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT SE SI SK TR
DOCDB simple family (publication)
WO 03075031 A1 20030912; AU 2003212262 A1 20030916; CA 2477476 A1 20030912; DE 10209177 A1 20030918; EP 1481258 A1 20041201; JP 2005526963 A 20050908; US 2005153358 A1 20050714
DOCDB simple family (application)
EP 0301825 W 20030222; AU 2003212262 A 20030222; CA 2477476 A 20030222; DE 10209177 A 20020301; EP 03708123 A 20030222; JP 2003573438 A 20030222; US 50614405 A 20050316