EP 1488446 A2 20041222 - METHOD AND SYSTEM FOR HIGH-THROUGHPUT QUANTITATION OF SMALL MOLECULES USING LASER DESORPTION AND MULTIPLE-REACTION-MONITORING
Title (en)
METHOD AND SYSTEM FOR HIGH-THROUGHPUT QUANTITATION OF SMALL MOLECULES USING LASER DESORPTION AND MULTIPLE-REACTION-MONITORING
Title (de)
VERFAHREN UND VORRICHTUNG ZUR HOCHDURCHSATZBESTIMMUNG KLEINER MOLEKÜLE MITTELS MATRIXGESTÜTZTER LASERDESORPTION UND MEHRFACHREAKTIONSÜBERWACHUNG
Title (fr)
PROCEDE ET SYSTEME DE QUANTIFICATION A HAUT RENDEMENT DE PETITES MOLECULES RECOURANT A LA DESORPTION LASER ET AU SUIVI DE REACTIONS MULTIPLES
Publication
Application
Priority
- IB 0301915 W 20030327
- US 36819502 P 20020328
Abstract (en)
[origin: WO03083448A2] A mass spectrometry quantitation technique enables high-throughput quantitation of small molecules using a laser-desorption (e.g., MALDI) ion source coupled to a triplequadrupole mass analyzer. The ions generated from the ion source are collisionally damped/cooled, and then quantitatively analyzed using the triple-quadrupole analyzer operated in the multiple-reaction-monitoring (MRM) mode. Significantly improved measurement sensitivity is obtained by applying laser pulses to the ion source at a high pulse rate of about SOOHz or higher. This allows the data acquisition to be performed rapidly, and the speed of about one second for each sample point on the ion source target has been achieved.
IPC 1-7
IPC 8 full level
G01N 27/62 (2006.01); B01D 59/44 (2006.01); G01N 21/00 (2006.01); G01N 27/64 (2006.01); H01J 49/00 (2006.01); H01J 49/10 (2006.01); H01J 49/16 (2006.01); H01J 49/40 (2006.01); H01J 49/42 (2006.01)
CPC (source: EP US)
H01J 49/0031 (2013.01 - EP US); H01J 49/004 (2013.01 - EP US); H01J 49/0481 (2013.01 - EP US); H01J 49/164 (2013.01 - EP US); H01J 49/4215 (2013.01 - EP US)
Citation (search report)
See references of WO 03083448A2
Citation (examination)
- US 2002030159 A1 20020314 - CHERNUSHEVICH IGOR [CA], et al
- DE HOFFMANN E: "Tandem mass spectrometry: A primer", JOURNAL OF MASS SPECTROMETRY, WILEY, CHICHESTER, GB LNKD- DOI:10.1002/(SICI)1096-9888(199602)31:2<,129::AID-JMS305>,3.0.CO,2-T, vol. 31, no. 2, 1 January 1996 (1996-01-01), pages 129 - 137, XP007903162, ISSN: 1076-5174
- COHEN L H ET AL: "Small molecule analysis by MALDI mass spectrometry", ANALYTICAL AND BIOANALYTICAL CHEMISTRY 2002 SPRINGER VERLAG DE, vol. 373, no. 7, 12 June 2002 (2002-06-12), pages 571 - 586, XP007913578, DOI: DOI:10.1007/S00216-002-1321-Z
- DUNCAN MARK W ET AL: "Quantitative analysis of low molecular weight compounds of biological interest by matrix-assisted laser desorption ionization", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 7, no. 12, 1993, pages 1090 - 1094, XP007913579, ISSN: 0951-4198
- MUDDIMAN DAVID C ET AL: "Quantitative measurement of cyclosporin A in blood by time-of-flight mass spectrometry", ANALYTICAL CHEMISTRY, vol. 66, no. 14, 1994, pages 2362 - 2368, XP007913580, ISSN: 0003-2700
- GUSEV ARKADY I ET AL: "A quantitative study of in vitro hepatic metabolism of Tacrolimus (FK506) using secondary ion and matrix-assisted laser desorption/ionization mass spectrometry", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, HEYDEN, LONDON, GB, vol. 10, no. 10, 1 January 1996 (1996-01-01), pages 1215 - 1218, XP007917448, ISSN: 0951-4198
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 03083448 A2 20031009; WO 03083448 A3 20031231; AU 2003230093 A1 20031013; AU 2003230093 A8 20031013; CA 2477835 A1 20031009; CA 2477835 C 20111122; EP 1488446 A2 20041222; JP 2005521874 A 20050721; JP 2009282038 A 20091203; US 2003213901 A1 20031120; US 2006151691 A1 20060713; US 6930305 B2 20050816
DOCDB simple family (application)
IB 0301915 W 20030327; AU 2003230093 A 20030327; CA 2477835 A 20030327; EP 03722936 A 20030327; JP 2003580837 A 20030327; JP 2009170646 A 20090721; US 40032203 A 20030327; US 50583705 A 20050705