EP 1490488 A2 20041229 - PURIFICATION METHODS FOR OLIGONUCLEOTIDES AND THEIR ANALOGS
Title (en)
PURIFICATION METHODS FOR OLIGONUCLEOTIDES AND THEIR ANALOGS
Title (de)
METHODE ZUR REINIGUNG VON OLIGONUKLEOTIDEN UND IHRER ANALOGE
Title (fr)
PROCEDES DE PURIFICATION POUR DES OLIGONUCLEOTIDES ET LEURS ANALOGUES
Publication
Application
Priority
- GB 0301161 W 20030319
- US 36706002 P 20020321
Abstract (en)
[origin: WO03080834A2] The present invention discloses methods for separating oligonucleotides from impurities. In the methods of the invention, a target oligonucleotide, in a mixture comprising the target oligonucleotide and an impurity, is separated from the impurity using a titratable anion exchange composition. The target oligonucleotide is bound to the titratable anion exchange composition and an eluting solution which increases in pH over time is passed through the titratable anion exchange composition with the target oligonucleotide bound thereon. Preferably, the eluting solution does not substantially increase its salt concentration. The target oligonucleotide is eluted and thereby separated from the impurity which either elutes at a lower pH or a higher pH than the target oligonucleotide.
IPC 1-7
IPC 8 full level
B01J 41/10 (2006.01); B01J 41/12 (2006.01); B01J 41/20 (2006.01); B01J 49/00 (2006.01); C07H 21/04 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); G01N 30/88 (2006.01)
CPC (source: EP KR)
C12N 15/10 (2013.01 - KR); C12N 15/101 (2013.01 - EP)
Citation (search report)
See references of WO 03080834A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 03080834 A2 20031002; WO 03080834 A3 20031231; AU 2003216830 A1 20031008; CA 2479901 A1 20031002; CN 1643145 A 20050720; EP 1490488 A2 20041229; JP 2005520547 A 20050714; KR 20040108672 A 20041224
DOCDB simple family (application)
GB 0301161 W 20030319; AU 2003216830 A 20030319; CA 2479901 A 20030319; CN 03806393 A 20030319; EP 03712366 A 20030319; JP 2003578560 A 20030319; KR 20047014747 A 20030319