Global Patent Index - EP 1490488 A2

EP 1490488 A2 2004-12-29 - PURIFICATION METHODS FOR OLIGONUCLEOTIDES AND THEIR ANALOGS

Title (en)

PURIFICATION METHODS FOR OLIGONUCLEOTIDES AND THEIR ANALOGS

Title (de)

METHODE ZUR REINIGUNG VON OLIGONUKLEOTIDEN UND IHRER ANALOGE

Title (fr)

PROCEDES DE PURIFICATION POUR DES OLIGONUCLEOTIDES ET LEURS ANALOGUES

Publication

EP 1490488 A2 (EN)

Application

EP 03712366 A

Priority

  • GB 0301161 W
  • US 36706002 P

Abstract (en)

[origin: WO03080834A2] The present invention discloses methods for separating oligonucleotides from impurities. In the methods of the invention, a target oligonucleotide, in a mixture comprising the target oligonucleotide and an impurity, is separated from the impurity using a titratable anion exchange composition. The target oligonucleotide is bound to the titratable anion exchange composition and an eluting solution which increases in pH over time is passed through the titratable anion exchange composition with the target oligonucleotide bound thereon. Preferably, the eluting solution does not substantially increase its salt concentration. The target oligonucleotide is eluted and thereby separated from the impurity which either elutes at a lower pH or a higher pH than the target oligonucleotide.

IPC 1-7 (main, further and additional classification)

C12N 15/10

IPC 8 full level (invention and additional information)

G01N 30/88 (2006.01); B01J 41/10 (2006.01); B01J 41/12 (2006.01); B01J 41/20 (2006.01); B01J 49/00 (2006.01); C07H 21/04 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01)

CPC (invention and additional information)

C12N 15/101 (2013.01)

Citation (search report)

See references of WO 03080834A3

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

EPO simple patent family

WO 03080834 A2 20031002; WO 03080834 A3 20031231; AU 2003216830 A1 20031008; CA 2479901 A1 20031002; CN 1643145 A 20050720; EP 1490488 A2 20041229; JP 2005520547 A 20050714; KR 20040108672 A 20041224

INPADOC legal status


2007-05-30 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20061213

2006-09-06 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20060803

2004-12-29 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20041021

2004-12-29 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

2004-12-29 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Countries: AL LT LV MK