EP 1497455 A2 20050119 - DETECTION OF NUCLEIC ACID SEQUENCES BY CLEAVAGE AND SEPARATION OF TAG-CONTAINING STRUCTURES
Title (en)
DETECTION OF NUCLEIC ACID SEQUENCES BY CLEAVAGE AND SEPARATION OF TAG-CONTAINING STRUCTURES
Title (de)
NACHWEIS VON NUKLEINSÄURESEQUENZEN DURCH SPALTUNG UND TRENNUNG TAGS ENTHALTENDER STRUKTUREN
Title (fr)
DETECTION DE SEQUENCES D'ACIDES NUCLEIQUES PAR CLIVAGE ET SEPARATION DE STRUCTURES MARQUEES
Publication
Application
Priority
- US 0235552 W 20021106
- US 33768601 P 20011109
Abstract (en)
[origin: WO03042657A2] The present invention is directed to a method of detecting pluralities of target nucleic acid sequences by forming and cleaving duplex structures with a pair of probes, one probe of each pair being labeled with an electrophoretic tag. Cleavage of the duplex structures releases electrophoretic tags that are then separated and identified to indicate the presence or quantity of the target sequences. The present invention is particularly useful in multiplex reactions wherein multiple target sequences are detected in one reaction. Kits useful in the detection of nucleic acids are also provided.
IPC 1-7
IPC 8 full level
G01N 27/447 (2006.01); C07H 19/10 (2006.01); C07H 19/20 (2006.01); C07H 21/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/34 (2006.01); C12Q 1/68 (2006.01); C40B 30/00 (2006.01)
CPC (source: EP)
C07H 19/10 (2013.01); C07H 19/20 (2013.01); C07H 21/00 (2013.01); C12Q 1/682 (2013.01); C12Q 1/6823 (2013.01)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR
DOCDB simple family (publication)
WO 03042657 A2 20030522; WO 03042657 A3 20041028; AU 2002360344 A1 20030526; CA 2465588 A1 20030522; EP 1497455 A2 20050119; EP 1497455 A4 20051228; JP 2005511033 A 20050428
DOCDB simple family (application)
US 0235552 W 20021106; AU 2002360344 A 20021106; CA 2465588 A 20021106; EP 02795593 A 20021106; JP 2003544441 A 20021106