EP 1509599 A1 20050302 - ALDEHYDE DEHYDROGENASE
Title (en)
ALDEHYDE DEHYDROGENASE
Title (de)
ALDEHYD-DEHYDROGENASE
Title (fr)
ALDEHYDE DESHYDROGENASE
Publication
Application
Priority
- EP 03740161 A 20030530
- EP 0305676 W 20030530
- EP 02012582 A 20020606
Abstract (en)
[origin: WO03104445A1] The present invention concerns a novel aldehyde dehydrogenase having the following physico-chemical properties: a molecular weight of 190,000 ± 15,000 Da which comprises a subunit structure of two alpha subunits and one beta subunit, or a molecular weight of 250,000 ± 20,000 Da which comprises a subunit structure of two alpha subunits and two beta subunits, in which the alpha subunit has a molecular weight of 75,000 ± 3,000 Da and the beta subunit has a molecular weight of 55,000 ± 2,000 Da; dehydrogenase activity on L-sorbosone, D-glucosone, D-glucose and D-xylose; utilizes as cofactor pyrroloquinoline quinone and heme c; has an optimum pH of from about 6.5 to about 8.0 for the production of vitamin C and an optimum pH of about 9.0 for the production of 2-keto-L-gulonic acid from L-sorbosone; and is inhibited by Co<2+>, Cu<2+>, Fe<3+>, Ni<2+>, Zn<2+>, Mg<2+>, monoiodoacetate, and sodium azide.
IPC 1-7
IPC 8 full level
C12N 9/02 (2006.01); C12P 1/04 (2006.01); C12P 7/58 (2006.01); C12P 7/60 (2006.01); C12P 17/04 (2006.01); C12R 1/02 (2006.01)
CPC (source: EP)
C12N 9/0008 (2013.01); C12P 7/60 (2013.01); C12P 17/04 (2013.01)
Citation (search report)
See references of WO 03104445A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 03104445 A1 20031218; AU 2003274096 A1 20031222; CN 1659275 A 20050824; EP 1509599 A1 20050302; JP 2005528917 A 20050929
DOCDB simple family (application)
EP 0305676 W 20030530; AU 2003274096 A 20030530; CN 03813023 A 20030530; EP 03740161 A 20030530; JP 2004511505 A 20030530