EP 1511842 A2 20050309 - METHOD FOR CREATING POLYNUCLEOTIDE MOLECULES
Title (en)
METHOD FOR CREATING POLYNUCLEOTIDE MOLECULES
Title (de)
VERFAHREN ZUR ERZEUGUNG VON POLYNUKLEOTIDMOLEKÜLEN
Title (fr)
PROCEDE DE PRODUCTION DE MOLECULES DE TYPE POLYNUCLEOTIDES
Publication
Application
Priority
- DE 10223057 A 20020524
- EP 0305308 W 20030521
Abstract (en)
[origin: WO03100058A2] The invention relates to methods for creating polynucleotide molecules with modified characteristics, said methods running through a cycle comprising the following steps:(a) provision of a double-stranded polynucleotide molecule or a population of double-stranded polynucleotide molecules, the individual polynucleotides of said population having at least one homologous sequence part and at least one heterologous sequence part,(b) creation of single-strand breaks in the double-stranded polynucleotide molecules, (c) nucleolytic degradation in the 5'} 3' direction starting from the single-strand breaks with simultaneous new synthesis in the 5' } 3' direction and shifting of the single-strand breaks in the direction of the 3' end, (d) production of single-stranded polynucleotide molecules, (e) production of partially double-stranded polynucleotide molecules of the single-stranded polynucleotide molecules provided in step (d), (f) template-directed nucleic acid synthesis starting from the partially double-stranded polynucleotide molecules produced in step (e), whereby steps (b) and (c) can be carried out in succession or simultaneously.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01)
CPC (source: EP KR)
C12N 15/10 (2013.01 - KR); C12N 15/1027 (2013.01 - EP); C12P 19/34 (2013.01 - EP KR)
Citation (search report)
See references of WO 03100058A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 03100058 A2 20031204; WO 03100058 A3 20040902; AU 2003240683 A1 20031212; CA 2485218 A1 20031204; CN 1656221 A 20050817; DE 10223057 A1 20031211; EP 1511842 A2 20050309; JP 2005529596 A 20051006; KR 20050004207 A 20050112; MX PA04011554 A 20050307
DOCDB simple family (application)
EP 0305308 W 20030521; AU 2003240683 A 20030521; CA 2485218 A 20030521; CN 03811927 A 20030521; DE 10223057 A 20020524; EP 03730082 A 20030521; JP 2004508297 A 20030521; KR 20047018944 A 20030521; MX PA04011554 A 20030521