EP 1511862 A4 20060118 - ELECTRICAL DETECTION OF DNA HYBRIDIZATION AND SPECIFIC BINDING EVENTS
Title (en)
ELECTRICAL DETECTION OF DNA HYBRIDIZATION AND SPECIFIC BINDING EVENTS
Title (de)
ELEKTRISCHER NACHWEIS VON DNA-HYBRIDISIERUNG UND SPEZIFISCHEN BINDUNGSEREIGNISSEN
Title (fr)
DETECTION ELECTRIQUE D'HYBRIDATION D'ADN ET D'AUTRES EVENEMENTS SPECIFIQUES DE LIAISON
Publication
Application
Priority
- US 0315498 W 20030514
- US 38044102 P 20020514
Abstract (en)
[origin: US2004014106A1] A method for detecting a target analyte having a first binding site and a second binding site. A substrate is provided having at least a first and a second patterned conductor, the first conductor being separated from the second conductor. The arrangement of the patterned conductors forms at least two substantially non-conducting gaps. The method may also include contacting to the substrate capture probes that bind specifically to the first binding site of the target analyte and providing electrically conductive nanoparticles having bound thereto binding sites that bind specifically to the second binding site of the target analyte. Then, contacting the substrate and the electrically conductive nanoparticles with the target analyte under hybridizing conditions will bind the target analyte to the substrate and to the electrically conductive nanoparticles. The electrically conductive nanoparticles between the conductors can thus be electrically detected. Detection can be improved by silver deposition of the nanoparticles.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01); G01N 33/543 (2006.01); G01R 15/12 (2006.01)
CPC (source: EP US)
C12Q 1/68 (2013.01 - EP US); C12Q 1/6825 (2013.01 - EP); C12Q 1/6834 (2013.01 - EP US); G01N 33/5438 (2013.01 - EP US); G01R 15/12 (2013.01 - EP US)
Citation (search report)
- [X] WO 0100876 A1 20010104 - MIRKIN CHAD A [US], et al
- [A] WO 9804740 A1 19980205 - UNIV NORTHWESTERN [US], et al
- [A] US 6342359 B1 20020129 - LEE WON YONG [KR], et al
- [X] PARK S-J ET AL: "ARRAY-BASED ELECTRICAL DETECTION OF DNA WITH NANOPARTICLE PROBES", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 295, 22 February 2002 (2002-02-22), pages 1503 - 1506, XP001160895, ISSN: 0036-8075
- See references of WO 2004042070A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
US 2004014106 A1 20040122; AU 2003299508 A1 20040607; CA 2484948 A1 20040521; EP 1511862 A2 20050309; EP 1511862 A4 20060118; JP 2006501486 A 20060112; WO 2004042070 A2 20040521; WO 2004042070 A3 20040826; WO 2004042070 A9 20050120
DOCDB simple family (application)
US 43775303 A 20030514; AU 2003299508 A 20030514; CA 2484948 A 20030514; EP 03799795 A 20030514; JP 2004549897 A 20030514; US 0315498 W 20030514