Global Patent Index - EP 1514216 A4

EP 1514216 A4 20100106 - GENERATION AND SELECTION OF PROTEIN LIBRARY IN SILICO

Title (en)

GENERATION AND SELECTION OF PROTEIN LIBRARY IN SILICO

Title (de)

ERZEUGUNG UND AUSWAHL EINER PROTEINBIBLIOTHEK IN SILICO

Title (fr)

PRODUCTION ET SELECTION D'UNE BANQUE DE PROTEINES DANS DE LA SILICE

Publication

EP 1514216 A4 20100106 (EN)

Application

EP 03755415 A 20030520

Priority

  • US 0316037 W 20030520
  • US 15317602 A 20020520
  • US 15315902 A 20020520

Abstract (en)

[origin: WO03099999A2] The present invention provides a methodology for efficiently generating and screening protein libraries for optimized proteins with desirable biological functions, such as improved binding affinity towards biologically and/or therapeutically important target molecules. The process is carried out computationally in a high throughput manner by mining the ever-expanding databases of protein sequences of all organisms, especially human. In one embodiment, a method for constructing a library of designed proteins, comprising the steps of: providing an amino acid sequence derived from a lead protein, the amino acid sequence being designated as a lead sequence; comparing the lead sequence with a plurality of tester protein sequences; and selecting from the plurality of tester protein sequences at least two peptide segments that have at least 15% sequence identity with the lead sequence, the selected peptide segments forming a hit library; and forming a library of designed proteins by substituting the lead sequence with the hit library. The library of designed proteins can be expressed in vitro or in vivo to produce a library of recombinant proteins that can be screened for novel or improved function(s) over the lead protein, such as an antibody against therapeutically important target.

IPC 1-7

G06F 19/00

IPC 8 full level

C07K 16/00 (2006.01); C12N 15/09 (2006.01); C07K 16/22 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01)

CPC (source: EP US)

C07K 16/00 (2013.01 - EP); C07K 16/22 (2013.01 - EP); G16B 20/30 (2019.02 - EP US); G16B 30/00 (2019.02 - EP US); G16B 30/10 (2019.02 - EP US); G16B 35/00 (2019.02 - EP); G16B 35/20 (2019.02 - EP US); G16C 20/60 (2019.02 - EP); C07K 2317/24 (2013.01 - EP); C07K 2317/622 (2013.01 - EP); C07K 2319/00 (2013.01 - EP); G16B 20/00 (2019.02 - EP); G16B 20/50 (2019.02 - EP US)

Citation (search report)

[PX] WO 02084277 A1 20021024 - ABMAXIS INC [US], et al

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

DOCDB simple family (publication)

WO 03099999 A2 20031204; WO 03099999 A3 20040916; AU 2003248548 A1 20031212; AU 2003248548 B2 20100311; CA 2485732 A1 20031204; CN 1672160 A 20050921; CN 1672160 B 20100609; EP 1514216 A2 20050316; EP 1514216 A4 20100106; JP 2005526518 A 20050908; SG 135053 A1 20070928

DOCDB simple family (application)

US 0316037 W 20030520; AU 2003248548 A 20030520; CA 2485732 A 20030520; CN 03817360 A 20030520; EP 03755415 A 20030520; JP 2004508241 A 20030520; SG 2006004352 A 20030520