Global Patent Index - EP 1517702 A4

EP 1517702 A4 20060503 - IMMUNOGENIC HBc CHIMER PARTICLES STABILIZED WITH AN N-TERMINAL CYSTEINE

Title (en)

IMMUNOGENIC HBc CHIMER PARTICLES STABILIZED WITH AN N-TERMINAL CYSTEINE

Title (de)

MIT EINEM N-TERMINALEN CYSTEIN STABILISIERTE IMMUNOGENE HBc-CHIMÄRE-PARTIKEL

Title (fr)

PARTICULES CHIMERIQUES HBC IMMUNOGENES STABILISEES AVEC UNE CYSTEINE D'EXTREMITE N-TERMNALE

Publication

EP 1517702 A4 20060503 (EN)

Application

EP 03709214 A 20030221

Priority

  • US 0305196 W 20030221
  • US 8029902 A 20020221
  • US 8201402 A 20020221
  • US 27461602 A 20021021

Abstract (en)

[origin: WO03102165A2] A chimeric, carboxy-terminal truncated hepatitis B virus nucleocapsid protein (HBc) is disclosed that is engineered for both enhanced stability of self-assembled particles and the display of an immunogenic B cell or T cell epitope, or both, such as a B cell epitope polypeptide of the influenza M2 protein and a T cell epitope of the influenza NP protein. An immunogenic epitope is peptide-bonded to one or more of the N-terminus, in the immunogenic loop or at the C-terminus of HBc, whereas the enhanced stability of self-assembled particles is obtained by the presence of at least one heterologous cysteine residue near the amino-terminus of the chimer molecule. Methods of making and using the chimers are also disclosed.

IPC 8 full level

C12Q 1/70 (2006.01); A61K 39/015 (2006.01); A61K 39/145 (2006.01); A61K 39/29 (2006.01); A61K 39/385 (2006.01); C07K 14/02 (2006.01)

CPC (source: EP)

A61K 39/015 (2013.01); A61K 39/12 (2013.01); A61K 39/145 (2013.01); C07K 14/005 (2013.01); A61K 2039/5258 (2013.01); A61K 2039/543 (2013.01); A61K 2039/55505 (2013.01); A61K 2039/55566 (2013.01); A61K 2039/55572 (2013.01); A61K 2039/57 (2013.01); C07K 2319/00 (2013.01); C12N 2730/10122 (2013.01); C12N 2730/10134 (2013.01); C12N 2760/16134 (2013.01); Y02A 50/30 (2017.12)

Citation (search report)

  • [Y] WO 9940934 A1 19990819 - IMMUNE COMPLEX CORP [US]
  • [Y] WO 9957289 A2 19991111 - UNIV MICHIGAN STATE [US], et al
  • [PX] WO 0214478 A2 20020221 - APOVIA INC [US]
  • [Y] ULRICH R ET AL: "CORE PARTICLES OF HEPATITIS B VIRUS AS CARRIER FOR FOREIGN EPITOPES", ADVANCES IN VIRUS RESEARCH, SAN DIEGO, CA, US, vol. 50, 1998, pages 141 - 182, XP000856161
  • [Y] KRATZ P A ET AL: "NATIVE DISPLAY OF COMPLETE FOREIGN PROTEIN DOMAINS ON THE SURFACE OF HEPATITIS B VIRUS CAPSIDS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 96, 1999, pages 1915 - 1920, XP000910194, ISSN: 0027-8424
  • [Y] ZHOU S ET AL: "Cys residues of the hepatitis B virus capsid protein are not essential for the assembly of viral core particles but can influence their stability", JOURNAL OF VIROLOGY, NEW YORK, US, US, vol. 66, no. 9, September 1992 (1992-09-01), pages 5393 - 5398, XP002961917, ISSN: 0022-538X
  • [A] SEIFER M ET AL: "Stability governs the apparent expression of particulate hepatitis e antigen by mutant hepatitis B virus core particles", VIROLOGY, ACADEMIC PRESS,ORLANDO, US, vol. 196, no. 1, September 1993 (1993-09-01), pages 70 - 78, XP002961918, ISSN: 0042-6822
  • See references of WO 03102165A2

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT SE SI SK TR

DOCDB simple family (publication)

WO 03102165 A2 20031211; WO 03102165 A3 20050106; AU 2003213168 A1 20031219; AU 2003213168 A8 20031219; EP 1517702 A2 20050330; EP 1517702 A4 20060503

DOCDB simple family (application)

US 0305196 W 20030221; AU 2003213168 A 20030221; EP 03709214 A 20030221