EP 1525266 A1 20050427 - SITE-SPECIFIC LABELLING OF PROTEINS USING CYANINE DYE REPORTERS
Title (en)
SITE-SPECIFIC LABELLING OF PROTEINS USING CYANINE DYE REPORTERS
Title (de)
ORTSSPEZIFISCHE MARKIERUNG VON PROTEINEN MIT CYANINFARBSTOFFREPORTERN
Title (fr)
MARQUAGE SPECIFIQUE DE PROTEINES EN FONCTION DU SITE AU MOYEN DE COLORANTS DE SIGNALISATION A BASE DE CYANINE
Publication
Application
Priority
- GB 0303196 W 20030728
- GB 0217556 A 20020730
- US 24133302 A 20020911
Abstract (en)
[origin: WO2004011556A1] Disclosed are compounds of formula (I):in which D is a dye selected from a cyanine dye or a derivative thereof; B is an affinity tag;F comprises a target bonding group selected from a carboxylic acid thioester group and a 1,2-aminothiol group; M is a group adapted for attaching to F; and L<1> and L<2> each independently comprise a group containing from 1 to 40 linked atoms selected from carbon atoms which may optionally include one or more groups selected from -NR'- , -O- , -CH=CH- , -CO-NH- and phenylenyl groups, where R' is selected from hydrogen and C1 - C4 alkyl. The invention also relates to methods that afford direct attachment of the cyanine dye reporter group to either the N-terminus or C-terminus of a synthetic or recombinant peptide or protein, and their derivatives, in a site-specific manner, coupled with purification of the resultant labelled molecule.
IPC 1-7
IPC 8 full level
C07D 209/14 (2006.01); C07D 403/14 (2006.01); C07D 495/04 (2006.01); C07K 1/13 (2006.01); C07K 1/22 (2006.01); C09B 23/00 (2006.01); C09B 23/08 (2006.01); G01N 33/58 (2006.01)
CPC (source: EP)
C09B 23/083 (2013.01); G01N 33/582 (2013.01)
Citation (search report)
See references of WO 2004011556A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2004011556 A1 20040205; AU 2003246957 A1 20040216; CA 2493309 A1 20040205; EP 1525266 A1 20050427; JP 2005534739 A 20051117
DOCDB simple family (application)
GB 0303196 W 20030728; AU 2003246957 A 20030728; CA 2493309 A 20030728; EP 03771163 A 20030728; JP 2004523938 A 20030728