EP 1534864 A2 20050601 - METHOD FOR THE DETECTION OF NUCLEIC ACID SEQUENCES BY MEANS OF CRACKABLE PROBE MOLECULES
Title (en)
METHOD FOR THE DETECTION OF NUCLEIC ACID SEQUENCES BY MEANS OF CRACKABLE PROBE MOLECULES
Title (de)
VERFAHREN ZUM NACHWEIS VON NUKLEINSÄURESEQUENZEN MITTELS SPALTBARER SONDENMOLEKÜLE
Title (fr)
PROCEDE DE DETECTION DE SEQUENCES D'ACIDES NUCLEIQUES AU MOYEN DE MOLECULES SONDE COMPORTANT UNE LIAISON CASSABLE
Publication
Application
Priority
- DE 0302936 W 20030901
- DE 10240746 A 20020901
Abstract (en)
[origin: WO2004022779A2] The invention relates to a method for detecting nucleic acid sequences, which is characterized by the fact that the following steps are carried out: a) at least one nucleic acid is bound onto a solid phase; b) probe molecules which are provided with a crackable bond and a probe molecule-specific mass marker are hybridized in a sequence-specific manner to the nucleic acid; c) the probe molecules that are not hybridized are removed; d) the hybridized probe molecules are brought into contact with a matrix which cracks said crackable bonds and is used as a matrix in a MALDI mass spectrometer; e) the mass markers are detected in the positions in which the nucleic acid is bound.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/6816 (2013.01 - EP US)
Citation (search report)
See references of WO 2004022779A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2004022779 A2 20040318; WO 2004022779 A3 20040603; AU 2003266197 A1 20040329; DE 10240746 A1 20040318; EP 1534864 A2 20050601; US 2006166201 A1 20060727
DOCDB simple family (application)
DE 0302936 W 20030901; AU 2003266197 A 20030901; DE 10240746 A 20020901; EP 03793600 A 20030901; US 52627705 A 20050919