Global Patent Index - EP 1539997 A4

EP 1539997 A4 20070425 - HIGH-THROUGHPUT ASSAY OF HEMATOPOIETIC STEM AND PROGENITOR CELL PROLIFERATION

Title (en)

HIGH-THROUGHPUT ASSAY OF HEMATOPOIETIC STEM AND PROGENITOR CELL PROLIFERATION

Title (de)

ASSAY MIT HOHEM DURCHSATZ FÜR DIE PROLIFERATION HÄMATOPOETISCHER STAMM- UND VORLÄUFERZELLEN

Title (fr)

DOSAGE A HAUT RENDEMENT DE PROLIFERATION DE SOUCHES HEMATOPOIETIQUES ET DE CELLULES PROGENITRICES

Publication

EP 1539997 A4 20070425 (EN)

Application

EP 03793209 A 20030821

Priority

  • US 0326167 W 20030821
  • US 40497202 P 20020821

Abstract (en)

[origin: WO2004018996A2] The present invention relates generally to kits that provide reagent mixes and instructions for the use thereof, in performing high-throughput assay methods that determine the proliferative status of isolated target cell populations. The methods measure the luminescent output derived from the intracellular ATP content of incubated target cells, and correlate the luminescence with the proliferative status of the cells. The present invention further relates to kits that provide reagent mixes and instructions for high-throughput assays methods for screening compounds that may modulate the proliferative status of a target cell population. The kits of the present invention and methods therein described may be used for determining the proliferative status of any isolated cell line or type. The kits and methods of the present invention address the need for rapid assays that determine the proliferative status of isolated hematopoietic stem and progenitor cells and of subpopulations of differentiated cells thereof.

IPC 8 full level

G01N 33/50 (2006.01); C12Q 1/66 (2006.01)

CPC (source: EP)

C12Q 1/66 (2013.01); G01N 33/5005 (2013.01); G01N 33/5088 (2013.01)

Citation (search report)

  • [PX] WO 03004995 A2 20030116 - RICH IVAN N [US]
  • [PY] RICH IVAN N ET AL: "HALO - A Multifunctional Colony-Forming-Based Assay Platform for Drug Development and Basic and Clinical Research.", BLOOD, vol. 100, no. 11, 16 November 2002 (2002-11-16), & 44TH ANNUAL MEETING OF THE AMERICAN SOCIETY OF HEMATOLOGY; PHILADELPHIA, PA, USA; DECEMBER 06-10, 2002, pages Abstract No. 2434, XP009048843, ISSN: 0006-4971
  • [Y] DATABASE WPI Week 1998, Derwent World Patents Index; AN 1998-297920, XP002424190, "Culture method kit haematopoietic stem cell culture cell adhesive culture vessel presence stem cell growth factor"
  • [Y] CROUCH S P M ET AL: "THE USE OF ATP BIOLUMINESCENCE AS A MEASURE OF CELL PROLIFERATION AND CYTOTOXICITY", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 160, no. 1, 1993, pages 81 - 88, XP002059949, ISSN: 0022-1759
  • [Y] SOTTONG P R ET AL: "Measurement of T-lymphocyte responses in whole-blood cultures using newly synthesized DNA and ATP.", CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY. MAR 2000, vol. 7, no. 2, March 2000 (2000-03-01), pages 307 - 311, XP002331295, ISSN: 1071-412X
  • [Y] BULANOVA E G ET AL: "Bioluminescent assay for human lymphocyte blast transformation", IMMUNOLOGY LETTERS, vol. 46, no. 1-2, 1995, pages 153 - 155, XP009048894, ISSN: 0165-2478
  • [Y] KATAYAMA YOSHIO ET AL: "Replating potential of colony-forming units of granulocytes/macrophages (CFU-GM) expanded ex vivo by stem cell factor, interleukin (IL)-3, IL-6, granulocyte colony-stimulating factor, erythropoietin with or without thrombopoietin", INTERNATIONAL JOURNAL OF HEMATOLOGY, vol. 68, no. 2, August 1998 (1998-08-01), pages 157 - 168, XP009048892, ISSN: 0925-5710
  • [Y] FUJISAKI T ET AL: "Rapid differentiation of a rare subset of adult human lin(-)CD34(-)CD38(-) cells stimulated by multiple growth factors in vitro.", BLOOD 15 SEP 1999, vol. 94, no. 6, 15 September 1999 (1999-09-15), pages 1926 - 1932, XP002424141, ISSN: 0006-4971
  • [Y] GABBIANELLI M ET AL: "Multi-level effects of flt3 ligand on human hematopoiesis: expansion of putative stem cells and proliferation of granulomonocytic progenitors/monocytic precursors.", BLOOD 1 SEP 1995, vol. 86, no. 5, 1 September 1995 (1995-09-01), pages 1661 - 1670, XP002424142, ISSN: 0006-4971
  • [Y] BALLMAIER M ET AL: "C-mpl mutations are the cause of congenital amegakaryocytic thrombocytopenia", BLOOD, W.B.SAUNDERS COMPANY, ORLANDO, FL, US, vol. 97, no. 1, 2001, pages 139 - 146, XP002980535, ISSN: 0006-4971
  • [Y] BRADLEY T R ET AL: "THE EFFECT OF OXYGEN TENSION ON HAEMOPOIETIC AND FIBROBLAST CELL PROLIFERATION IN VITRO", JOURNAL OF CELLULAR PHYSIOLOGY, LISS, NEW YORK, NY, US, vol. 97, no. 3 PT 2 SUPPL1, December 1978 (1978-12-01), pages 517 - 522, XP009080296, ISSN: 0021-9541
  • [Y] RICH I N ET AL: "THE EFFECT OF REDUCED OXYGEN TENSION ON COLONY FORMATION OF ERYTHROPOIETIC CELLS IN VITRO", BRITISH JOURNAL OF HAEMATOLOGY, OXFORD, GB, vol. 52, no. 4, December 1982 (1982-12-01), pages 579 - 588, XP009080297, ISSN: 0007-1048 & WO 9821313 A1 19980522 - OTSUKA PHARMA CO LTD [JP], et al
  • See references of WO 2004018996A2

Citation (examination)

  • LEMOLI ROBERTO M ET AL: "Interleukin-9 stimulates the proliferation of human myeloid leukemic cells", BLOOD, vol. 87, no. 9, 1996, pages 3852 - 3859, ISSN: 0006-4971
  • SHIOHARA MASAAKI ET AL: "24-Oxo metabolites of vitamin D3 analogues: Disassociation of their prominent antileukemic effects from their lack of calcium modulation", CANCER RESEARCH, vol. 61, no. 8, 15 April 2001 (2001-04-15), pages 3361 - 3368, ISSN: 0008-5472

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

DOCDB simple family (publication)

WO 2004018996 A2 20040304; WO 2004018996 A3 20040610; AU 2003258319 A1 20040311; AU 2003258319 B2 20090917; CA 2496251 A1 20040304; EP 1539997 A2 20050615; EP 1539997 A4 20070425

DOCDB simple family (application)

US 0326167 W 20030821; AU 2003258319 A 20030821; CA 2496251 A 20030821; EP 03793209 A 20030821