EP 1540330 A4 20071017 - METHOD AND STAINING REAGENTS FOR STAINING HEMATOLOGY SAMPLES IN AN AUTOMATED STAINING APPARATUS
Title (en)
METHOD AND STAINING REAGENTS FOR STAINING HEMATOLOGY SAMPLES IN AN AUTOMATED STAINING APPARATUS
Title (de)
VERFAHREN UND FÄRBEMITTEL ZUM FÄRBEN VON HÄMATOLOGIEPROBEN IN EINER AUTOMATISIERTEN FÄRBEVORRICHTUNG
Title (fr)
PROCEDE ET REACTIFS DE COLORATION POUR COLORER DES ECHANTILLONS HEMATOLOGIQUES DANS UN APPAREIL DE COLORATION AUTOMATIQUE
Publication
Application
Priority
- US 0327030 W 20030829
- US 23247402 A 20020829
Abstract (en)
[origin: US2004043495A1] Automated staining equipment that can mix reagents is used to spray a Romanowsky stain onto slide mounted specimens which are then briefly centrifuged. The centrifugation step removes excess stain leaving only a thin film. Depending on the time of the centrifugation step, most of the organic solvent and part of the water in the stain are evaporated by airflow through the equipment. This greatly accelerates the staining reaction and preserves water soluble structures such as the granules in basophilic leukocytes. For optimal performance, this staining procedure requires a thiazin-eosin stain with about 90% to about 40% organic solvent, such as methanol, and only about 10% to about 60% water. This is a unique staining reagent in Romanowsky staining.
IPC 1-7
IPC 8 full level
G01N 1/30 (2006.01); G01N 1/31 (2006.01); G01N 35/00 (2006.01)
CPC (source: EP US)
G01N 1/30 (2013.01 - EP US); G01N 1/312 (2013.01 - EP US); G01N 2035/00495 (2013.01 - EP US); Y10S 436/80 (2013.01 - EP US); Y10T 436/10 (2015.01 - EP US); Y10T 436/107497 (2015.01 - EP US); Y10T 436/108331 (2015.01 - EP US); Y10T 436/11 (2015.01 - EP US); Y10T 436/111666 (2015.01 - EP US); Y10T 436/112499 (2015.01 - EP US)
Citation (search report)
- [XY] US 4595524 A 19860617 - YIP KIN F [US], et al
- [Y] WO 9963324 A1 19991209 - CELLAVISION AB [SE], et al
- [DY] US 5009185 A 19910423 - STOKES BARRY O [US], et al
- [XA] US 4363632 A 19821214 - CUSTARD ELIZABETH, et al
- [X] FR 2402204 A1 19790330 - TRAN THAI THANH [FR]
- [XY] MARSHALL P N ET AL: "Microspectrophotometric studies of Romanowsky stained blood cells. III. The action of methylene blue and azure B.", STAIN TECHNOLOGY JAN 1984, vol. 59, no. 1, January 1984 (1984-01-01), pages 17 - 36, XP008083479, ISSN: 0038-9153
- See references of WO 2004020995A1
Citation (examination)
US 3995022 A 19761130 - HEANLEY CHARLES PETER, et al
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
US 2004043495 A1 20040304; US 6858432 B2 20050222; AU 2003268247 A1 20040319; EP 1540330 A1 20050615; EP 1540330 A4 20071017; US 2005148083 A1 20050707; US 7303920 B2 20071204; WO 2004020995 A1 20040311
DOCDB simple family (application)
US 23247402 A 20020829; AU 2003268247 A 20030829; EP 03749201 A 20030829; US 0327030 W 20030829; US 6102305 A 20050218