Global Patent Index - EP 1546118 A4

EP 1546118 A4 20100804 - COMPOSITIONS AND METHODS FOR DETECTION AND ISOLATION OF PHOSPHORYLATED MOLECULES

Title (en)

COMPOSITIONS AND METHODS FOR DETECTION AND ISOLATION OF PHOSPHORYLATED MOLECULES

Title (de)

ZUSAMMENSETZUNGEN UND VERFAHREN ZUM NACHWEIS UND ZUR ISOLIERUNG PHOSPHORILIERTER MOLEK LE

Title (fr)

COMPOSITIONS ET METHODES DE DETECTION ET D'ISOLATION DE MOLECULES PHOSPHORYLEES

Publication

EP 1546118 A4 20100804 (EN)

Application

EP 03799756 A 20030502

Priority

  • US 0313765 W 20030502
  • US 37773302 P 20020503
  • US 39305902 P 20020628
  • US 40725502 P 20020830
  • US 44025203 P 20030114

Abstract (en)

[origin: US2004038306A1] The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

IPC 1-7

C07D 239/88; C07D 277/62; C07D 311/82; C07D 417/02; C07D 495/04; C07F 5/02; C08B 37/04; G01N 33/53

IPC 8 full level

G01N 33/531 (2006.01); B01J 20/281 (2006.01); C12Q 1/42 (2006.01); C12Q 1/48 (2006.01); C12Q 1/68 (2006.01); G01N 30/88 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01)

CPC (source: EP US)

G01N 33/50 (2013.01 - EP US); G01N 33/5008 (2013.01 - EP US); G01N 33/502 (2013.01 - EP US); G01N 33/5091 (2013.01 - EP US); G01N 2333/4709 (2013.01 - EP US)

Citation (search report)

  • [X] US 2002034766 A1 20020321 - HUANG WEI [US], et al
  • [X] US 5980862 A 19991109 - MEADE THOMAS [US], et al
  • [X] SHIHUA LI ET AL: "Iron(III)-Immobilized Metal Ion Affinity Chromatography and Mass Spectrometry for the Purification and Characterization of Synthetic Phosphopeptides", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS INC, NEW YORK LNKD- DOI:10.1006/ABIO.1999.4060, vol. 270, 1 January 1999 (1999-01-01), pages 9 - 14, XP002975579, ISSN: 0003-2697
  • [X] ZHOU W ET AL: "Detection and sequencing of phosphopeptides affinity bound to immobilized metal ion beads by matrix-assisted laser desorption/ionization mass spectrometry", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, ELSEVIER SCIENCE INC, US LNKD- DOI:10.1016/S1044-0305(00)00100-8, vol. 11, no. 4, 1 April 2000 (2000-04-01), pages 273 - 282, XP004191625, ISSN: 1044-0305
  • [Y] LATTANZIO F A ET AL: "The effect of pH on rate constants, ion selectivity and thermodynamic properties of fluorescent calcium and magnesium indicators", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US LNKD- DOI:10.1016/0006-291X(91)91966-G, vol. 177, no. 1, 31 May 1991 (1991-05-31), pages 184 - 191, XP024771051, ISSN: 0006-291X, [retrieved on 19910531]
  • [XY] KIMURA ET AL.: "1,2-Bis(o-aminophenoxy)ethane-N,N,N',N'-tetra-acetic acid derivatives on complexation with lanthanoid metal ions. Correlation between size of trapping hole and metal ion", HETEROATOM CHEMISTRY, vol. 6, no. 1, 1 January 1995 (1995-01-01), pages 57 - 62, XP007913100
  • See references of WO 2004042347A2

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

DOCDB simple family (publication)

US 2004038306 A1 20040226; US 7102005 B2 20060905; AU 2003299466 A1 20040607; CA 2483868 A1 20040521; CN 1665790 A 20050907; EP 1546118 A2 20050629; EP 1546118 A4 20100804; JP 2005539243 A 20051222; WO 2004042347 A2 20040521; WO 2004042347 A3 20050414

DOCDB simple family (application)

US 42819203 A 20030502; AU 2003299466 A 20030502; CA 2483868 A 20030502; CN 03815684 A 20030502; EP 03799756 A 20030502; JP 2004549877 A 20030502; US 0313765 W 20030502