EP 1546353 A2 20050629 - ALLELE SPECIFIC PRIMER EXTENSION
Title (en)
ALLELE SPECIFIC PRIMER EXTENSION
Title (de)
ALLELSPEZIFISCHE PRIMERVERLûNGERUNG
Title (fr)
EXTENSION D'AMORCE SPECIFIQUE A L'ALLELE
Publication
Application
Priority
- US 0327286 W 20030829
- US 40689402 P 20020829
Abstract (en)
[origin: WO2004020604A2] A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, an allele specific primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and optionally an enzyme having 3' ? 5' exonuclease activity when the primer is non-hydrolyzable, which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on such detection.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/42 (2006.01); C12Q 1/44 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/6869 (2013.01); C12Q 1/6823 (2013.01); C12Q 2600/156 (2013.01)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2004020604 A2 20040311; WO 2004020604 A3 20040812; AU 2003268322 A1 20040319; AU 2003268322 B2 20090716; CA 2496462 A1 20040311; CA 2496462 C 20140128; EP 1546353 A2 20050629; EP 1546353 A4 20060201; JP 2005537001 A 20051208; JP 4643263 B2 20110302
DOCDB simple family (application)
US 0327286 W 20030829; AU 2003268322 A 20030829; CA 2496462 A 20030829; EP 03749279 A 20030829; JP 2004532003 A 20030829