EP 1546353 A4 20060201 - ALLELE SPECIFIC PRIMER EXTENSION
Title (en)
ALLELE SPECIFIC PRIMER EXTENSION
Title (de)
ALLELSPEZIFISCHE PRIMERVERLûNGERUNG
Title (fr)
EXTENSION D'AMORCE SPECIFIQUE A L'ALLELE
Publication
Application
Priority
- US 0327286 W 20030829
- US 40689402 P 20020829
Abstract (en)
[origin: WO2004020604A2] A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, an allele specific primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and optionally an enzyme having 3' ? 5' exonuclease activity when the primer is non-hydrolyzable, which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on such detection.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/42 (2006.01); C12Q 1/44 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/6869 (2013.01); C12Q 1/6823 (2013.01); C12Q 2600/156 (2013.01)
Citation (search report)
- [PX] WO 03020891 A2 20030313 - AMERSHAM BIOSCIENCES CORP [US]
- [PX] WO 03020734 A2 20030313 - AMERSHAM BIOSCIENCES CORP [US]
- [PX] WO 03020984 A2 20030313 - AMERSHAM BIOSCIENCES CORP [US]
- [XY] WO 0244425 A2 20020606 - VISIGEN BIOTECHNOLOGIES INC [US]
- [XY] US 6255083 B1 20010703 - WILLIAMS JOHN G K [US]
- [X] WO 0194609 A1 20011213 - LI COR INC [US], et al
- [X] WO 0070073 A1 20001123 - CORNELL RES FOUNDATION INC [US]
- See references of WO 2004020604A2
Citation (examination)
- WO 0148235 A2 20010705 - ADVANCED RES & TECH INST [US]
- JP 2002101899 A 20020409 - HITACHI LTD
- US 5849487 A 19981215 - HASE TETSU [JP], et al & US 2003046928 A1 20030313 - VAN NIEUWSTADT MICHIEL JACQUES [US]
- SOOD ANUP ET AL: "Terminal phosphate-labeled nucleotides with improved substrate properties for homogeneous nucleic acid assays.", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2 MAR 2005 LNKD- PUBMED:15724985, vol. 127, no. 8, 2 March 2005 (2005-03-02), pages 2394 - 2395, XP002636624, ISSN: 0002-7863, DOI: 10.1021/JA043595X
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2004020604 A2 20040311; WO 2004020604 A3 20040812; AU 2003268322 A1 20040319; AU 2003268322 B2 20090716; CA 2496462 A1 20040311; CA 2496462 C 20140128; EP 1546353 A2 20050629; EP 1546353 A4 20060201; JP 2005537001 A 20051208; JP 4643263 B2 20110302
DOCDB simple family (application)
US 0327286 W 20030829; AU 2003268322 A 20030829; CA 2496462 A 20030829; EP 03749279 A 20030829; JP 2004532003 A 20030829