EP 1549923 B1 20121017 - FRAGMENTATION METHODS FOR MASS SPECTROMETRY
Title (en)
FRAGMENTATION METHODS FOR MASS SPECTROMETRY
Title (de)
FRAGMENTIERUNGSVERFAHREN FÜR DIE MASSENSPEKTROMETRIE
Title (fr)
PROCEDES DE FRAGMENTATION POUR SPECTROMETRIE DE MASSE
Publication
Application
Priority
- US 0317436 W 20030530
- US 38511302 P 20020531
Abstract (en)
[origin: WO03102545A2] Apparatus and methods are provided that enable the interaction of low-energy electrons and positrons with sample ions to facilitate electron capture dissociation (ECD) and positron capture dissociation (PCD), respectively, within multipole ion guide structures. It has recently been discovered that fragmentation of protonated ions of many biomolecules via ECD often proceeds along fragmentation pathways not accessed by other dissociation methods, leading to molecular structure information not otherwise easily obtainable. However, such analyses have been limited to expensive Fourier transform ion cyclotron resonance (FTICR) mass spectrometers; the implementation of ECD within commonly-used multipole ion guide structures is problematic due to the disturbing effects of RF fields within such devices. The apparatus and methods described herein successfully overcome such difficulties, and allow ECD (and PCD) to be performed within multipole ion guides, either alone, or in combination with conventional ion fragmentation methods. Therefore, improved analytical performance and functionality of mass spectrometers that utilize multipole ion guides are provided.
IPC 8 full level
H01J 49/06 (2006.01); G01N 1/00 (2006.01); H01J 37/12 (2006.01); H01J 49/42 (2006.01)
IPC 8 main group level
G01N (2006.01)
CPC (source: EP)
H01J 49/0054 (2013.01); H01J 49/063 (2013.01)
Citation (examination)
- SCOTT A. MCLUCKEY ET AL: "Gas-phase Ionization of Polyatomic Molecules via Interactions with Positrons", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 10, no. 3, 1 February 1996 (1996-02-01), pages 269 - 276, XP055024771, ISSN: 0951-4198, DOI: 10.1002/(SICI)1097-0231(199602)10:3<269::AID-RCM463>3.0.CO;2-R
- ROMAN A. ZUBAREV ET AL: "Electron Capture Dissociation of Multiply Charged Protein Cations. A Nonergodic Process", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 120, no. 13, 1 April 1998 (1998-04-01), pages 3265 - 3266, XP055024775, ISSN: 0002-7863, DOI: 10.1021/ja973478k
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 03102545 A2 20031211; WO 03102545 A3 20040506; AU 2003247472 A1 20031219; AU 2003247472 A8 20031219; CA 2487135 A1 20031211; CA 2487135 C 20090127; CA 2643534 A1 20031211; CA 2643534 C 20110802; EP 1549923 A2 20050706; EP 1549923 A4 20090325; EP 1549923 B1 20121017
DOCDB simple family (application)
US 0317436 W 20030530; AU 2003247472 A 20030530; CA 2487135 A 20030530; CA 2643534 A 20030530; EP 03756376 A 20030530