EP 1549958 A2 20050706 - MALDI-MATRIX
Title (en)
MALDI-MATRIX
Title (de)
MALDI-MATRIX
Title (fr)
MATRICE MALDI
Publication
Application
Priority
- DE 10238069 A 20020819
- EP 0308435 W 20030730
Abstract (en)
[origin: CA2496018A1] The invention relates to matrixes for ultraviolet matrix-assisted laser desorption-ionisation mass spectrometry made of the salt of an amine which reacts as a proton acceptor and an organic substance which reacts as a proto n donator. Either the amine or the organic substance absorbs UV light. Said matrixes are characterised in that they represent an ionic liquid at room temperature and in that the amine is selected from the group which is made u p of 3-aminoquinoline, pyridine, a primary amine whose N-Atom is bound to a phenyl radical or a straight or branched, saturated C1-C11-alkyl radical whi ch can be substituted by an OH-group, a secondary and tertiary amine whose N- Atoms are bound to two or three radicals which can be the same or different and represent a straight or branched, saturated C1-C8-alkyl radical which ca n be substituted by an OH-group and a phenyl radical, imidazole and the C and/ or N-alkylated imidazole derivatives and also characterised in that the organic substance is selected from the group which is made up of 2,5-dihydroxybenzoi c acid and the isomers thereof, 2-hydroxy-5-methoxy-benzoic acid and the isome rs thereof, picolinic-acid, 3-hydroxypicolinic acid, nicotinic acid, 5-chloro-2 - mercapto-benzo-thiazole, 6-aza-2-thiothymine, 2',4',6'-trihydroxyacetophenon e- monohydrate, 2',6'-di-hydroxyaceto-phenone, 9H-pyridol[3,4-b]indole, dithranole, trans-3-indolacrylic acid, osazones, ferulic acid, 2,5- dihydroxyacetophenone, 1-nitrocarbazole, 7-amino-4-methylcumarine, 2-(p- hdroxyphenylazo)-benzoic acid, 8-aminopyrene-2,3,4-trissulfonic acid, 2-[2E- 3- (4-tert-butylphenyl)-2-methylprop-2-enylidene]malonitril (DCTB), 4-methoxy-3 - hydroxycinnamic acid and 3,4-dihydroxycinnamic acid. Said liquid matrixes enable reproducible, error-free analysis values to be obtained. The combination of pure mass spectrometric analysis with additional knowledge of enzymatic reactions/modifications with the possible monitoring is also described.
IPC 1-7
IPC 8 full level
G01N 27/64 (2006.01); G01N 27/447 (2006.01); G01N 27/62 (2006.01); G01N 30/72 (2006.01); G01N 33/68 (2006.01); H01J 49/16 (2006.01)
CPC (source: EP US)
G01N 33/6848 (2013.01 - EP US); G01N 33/6851 (2013.01 - EP US); H01J 49/164 (2013.01 - EP US); Y10T 436/10 (2015.01 - EP US)
Citation (search report)
See references of WO 2004023147A2
Citation (examination)
- ARMSTRONG ET AL.: "Ionic liquids as matrixes for matrix-assited laser desorption/ionization mass spectrometry", ANAL. CHEM., vol. 73, no. 15, 2001, pages 3679 - 3686, XP001156233
- SZE & CHAN: "Formulation of matrix solutions for use in matrix-assited laser desorption/Ionization of biomolecules", J. AM. SOC. MASS SPECTROM., vol. 9, 1998, pages 166 - 174, XP004114925
- PFENNINGER ET AL.: "Matrix optimization for matrix-assited laser desorption/ionization mass spectrometry of oligosaccharides from human milk", J. MASS SPECTROM., vol. 34, 1999, pages 98 - 104, XP009023794, DOI: doi:10.1002/(SICI)1096-9888(199902)34:2<98::AID-JMS767>3.0.CO;2-N
- ANDERSON ET AL.: "Characterizing ionic liquids on the basis of multiple solvation interactions", J. AM. CHEM. SOC., vol. 124, 1 November 2002 (2002-11-01), pages 14247 - 14254, XP002267362
- KRAGL ET AL.: "Enzmy catalysis in ionic liquids.", CURRENT OPINION IN BIOTECHNOLOGY, vol. 13, 2002, pages 565 - 571
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
DE 10238069 A1 20040304; AU 2003258549 A1 20040329; CA 2496018 A1 20040318; CN 1675554 A 20050928; EP 1549958 A2 20050706; JP 2005536759 A 20051202; US 2005158863 A1 20050721; WO 2004023147 A2 20040318; WO 2004023147 A3 20050106
DOCDB simple family (application)
DE 10238069 A 20020819; AU 2003258549 A 20030730; CA 2496018 A 20030730; CN 03819761 A 20030730; EP 0308435 W 20030730; EP 03793665 A 20030730; JP 2004533295 A 20030730; US 50393504 A 20040816