EP 1554389 A2 20050720 - METHOD FOR MAKING RECOMBINANT PROTEINS
Title (en)
METHOD FOR MAKING RECOMBINANT PROTEINS
Title (de)
VERFAHREN ZUR HERSTELLUNG VON REKOMBINANTEN PROTEINEN
Title (fr)
PROCEDE DE FABRICATION DE PROTEINES RECOMBINANTES
Publication
Application
Priority
- DK 0300607 W 20030919
- DK PA200201384 A 20020920
- US 41656602 P 20021007
Abstract (en)
[origin: WO2004027072A2] The present invention relates to scaffold/matrix attachment regions isolated from hamster cells and their use for the industrial production of recombinant proteins and polypep-tides. In one embodiment the invention is related to a method for production of Factor VII polypeptides or Factor VII-related polypeptides comprising a) transfecting a mammalian cell with an expression vector comprising a nucleic acid molecule encoding Factor VII or a Factor VII-related polypeptide, expression control regions operatively linked to thereto and at least one S/MAR element; b) culturing the transfected cell under conditions for expression of Fac-tor VII or a Factor VII-related polypeptide and c) isolating the expressed polypeptide by suit-able means.
IPC 1-7
IPC 8 full level
C12N 9/64 (2006.01); C12N 15/85 (2006.01); C12P 21/02 (2006.01)
CPC (source: EP)
C12N 9/6437 (2013.01); C12N 15/85 (2013.01); C12P 21/02 (2013.01); C12Y 304/21021 (2013.01); C12N 2830/46 (2013.01); C12N 2840/20 (2013.01)
Citation (search report)
See references of WO 2004027072A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2004027072 A2 20040401; WO 2004027072 A3 20040513; AU 2003263161 A1 20040408; AU 2003263161 A8 20040408; EP 1554389 A2 20050720
DOCDB simple family (application)
DK 0300607 W 20030919; AU 2003263161 A 20030919; EP 03797190 A 20030919