EP 1556065 A1 20050727 - METHOD FOR ISOLATING SUBPOPULATIONS OF PROTEINS THAT ENGAGE IN PROTEIN-PROTEIN INTERACTIONS
Title (en)
METHOD FOR ISOLATING SUBPOPULATIONS OF PROTEINS THAT ENGAGE IN PROTEIN-PROTEIN INTERACTIONS
Title (de)
VERFAHREN ZUR ISOLIERUNG VON UNTERPOPULATIONEN VON PROTEINEN, DIE IN PROTEIN-PROTEIN-WECHSELWIRKUNGEN EINGREIFEN
Title (fr)
PROCEDE D'ISOLEMENT DE SOUS-POPULATIONS DE PROTEINES IMPLIQUEES DANS LES INTERACTIONS PROTEINE-PROTEINE
Publication
Application
Priority
US 0225845 W 20020814
Abstract (en)
[origin: WO2004019967A1] The invention provides a method for isolating and identifying proteins participating in protein-protein interactions in a complex mixture. The method uses a chemically reactive supporting matrix to isolate proteins that in turn non-covalently bind other proteins. The supporting matrix is isolated, and the non-covalently bound proteins are subsequently released for analysis. Because the proteins are accessible to chemical manipulation at both the binding and release steps, identification of the non-covalently bound proteins yields information on specific classes of interacting proteins, such as calcium-dependent or substrate-dependent protein interactions. This permits selection of a subpopulation of proteins from a complex mixture on the basis of specified interaction criteria. The method has the advantage of screening the entire proteome simultaneously, unlike two-hybrid systems or phage display methods which can only detect proteins binding to a single bait protein at a time. The method is applicable to the study of protein-protein interactions in biopsy and autopsy specimens, to the study of protein-protein interactions in the presence of signalling molecules, pharmacological agents or toxins, and for comparison of diseased and normal tissues or cancerous and untransformed cells.
IPC 1-7
A61K 38/00; C07K 1/00; C07K 14/00; C07K 17/00; G01N 33/53; G01N 33/543; G01N 33/566
IPC 8 full level
G01N 33/53 (2006.01); A61K 38/00 (2006.01); C07K 1/00 (2006.01); C07K 1/14 (2006.01); C07K 14/00 (2006.01); C07K 14/47 (2006.01); C07K 17/00 (2006.01); C07K 17/06 (2006.01); C40B 30/04 (2006.01); G01N 33/543 (2006.01); G01N 33/566 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP)
C07K 1/14 (2013.01); C40B 30/04 (2013.01); G01N 33/6842 (2013.01); G01N 33/6845 (2013.01)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR
DOCDB simple family (publication)
WO 2004019967 A1 20040311; AU 2002326644 A1 20040319; CA 2492324 A1 20040311; CN 1658892 A 20050824; EP 1556065 A1 20050727; EP 1556065 A4 20051019; JP 2005535727 A 20051124
DOCDB simple family (application)
US 0225845 W 20020814; AU 2002326644 A 20020814; CA 2492324 A 20020814; CN 02829465 A 20020814; EP 02761370 A 20020814; JP 2004532531 A 20020814