EP 1556500 A1 20050727 - SIMPLE ENZYMATIC PROCESS FOR PREPARING CEFAZOLIN
Title (en)
SIMPLE ENZYMATIC PROCESS FOR PREPARING CEFAZOLIN
Title (de)
EINFACHES ENZYMATISCHES VERFAHREN ZUR HERSTELLUNG VON CEFALOZOLIN
Title (fr)
PROCEDE ENZYMATIQUE SIMPLE DE PREPARATION DE CEFAZOLINE
Publication
Application
Priority
- EP 03773688 A 20031030
- EP 0312165 W 20031030
- EP 02380224 A 20021031
Abstract (en)
[origin: EP1416054A1] Preparing cefazolin (A) involves acylating 7-amino-3-[(methyl-1,3,4-thiadiazol-2-yl)thiomethyl]-3-cephem-4-carboxylic acid with tetrazole containing acetic acid in the presence of biocatalyst containing penicillin amidase at pH of 6-8 and at -20 - 40 [deg]C; and precipitating (A) by adding an acid to lower the pH below 3.5 at 0-20 [deg]C. Preparing cefazolin (A) involves acylating 7-amino-3-[(methyl-1,3,4-thiadiazol-2-yl)thiomethyl]-3-cephem-4-carboxylic acid (I) or its salt with tetrazole containing acetic acid of formula (II) in the presence of biocatalyst containing penicillin amidase (EC 3.5.1.11) (100 - 50000 units per liter) at pH of 6 - 8 and at -20 - 40 (preferably 0 - 10)[deg]C; and precipitating (A) by adding an acid to lower the pH below 3.5 at 0 - 20[deg]C. [Image] R : ester or amide of the acid. ACTIVITY : Antibiotic. No biological data is given. MECHANISM OF ACTION : None given.
IPC 1-7
IPC 8 full level
C07D 501/36 (2006.01); C12N 9/84 (2006.01); C12P 35/04 (2006.01)
CPC (source: EP)
C12P 35/04 (2013.01); C12Y 305/01011 (2013.01)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
EP 1416054 A1 20040506; EP 1416054 B1 20050817; AT E302283 T1 20050915; AU 2003282071 A1 20040525; DE 60205635 D1 20050922; EP 1556500 A1 20050727; WO 2004039997 A1 20040513
DOCDB simple family (application)
EP 02380224 A 20021031; AT 02380224 T 20021031; AU 2003282071 A 20031030; DE 60205635 T 20021031; EP 0312165 W 20031030; EP 03773688 A 20031030