EP 1563080 A1 20050817 - METHOD FOR EXPRESSING INDUCIBLE RNAI IN CELLS, NUCLEIC ACID MOLECULES THEREFOR AND CELLS TRANSFORMED BY SAID MOLECULES
Title (en)
METHOD FOR EXPRESSING INDUCIBLE RNAI IN CELLS, NUCLEIC ACID MOLECULES THEREFOR AND CELLS TRANSFORMED BY SAID MOLECULES
Title (de)
METHODE ZUR INDUZIERBAREN INDUKTION VON RNAI IN ZELLEN, DIE DAZU NOTWENDIGEN DNA MOLEKÜLE UND DURCH DIESE MOLEKÜLE TRANSFORMIERTE ZELLEN
Title (fr)
METHODE D'EXPRESSION INDUCTIBLE D'ARNI DANS DES CELLULES, LES MOLECULES D'ACIDE NUCLEIQUE POUR SA MISE EN OEUVRE ET LES CELLULES TRANSFORMEES PAR CES MOLECULES
Publication
Application
Priority
- FR 0303447 W 20031121
- FR 0214600 A 20021121
Abstract (en)
[origin: FR2847588A1] Method for expressing interfering RNA (I) in cells. Method for expressing interfering RNA (I) in cells comprises first introducing into eukaryotic cells a nucleic acid (II) comprising sense and antisense sequences (IIa, (IIb) for (I), under control of a single transcriptional promoter, where (IIa) and (IIb) are separated by a DNA sequence (IIc) that includes a transcription stop sequence (IId) and is flanked by lox sites. The lox sites are contacted with Cre to induce, by recombination, site-specific elimination of (IIc), including (IId), so that (IIa) and (IIb) are no longer separated, except by the residual lox site, so that transcription of complete (I) is possible, with the residual lox site forming a loop that connects the two RNA strands. Independent claims are also included for: (1) (II) as new compounds; and (2) cells, and cell lines, transfected with (II).
IPC 1-7
IPC 8 full level
C12N 15/12 (2006.01); C12N 15/85 (2006.01); A61K 38/00 (2006.01)
CPC (source: EP US)
C12N 15/85 (2013.01 - EP US); A61K 38/00 (2013.01 - EP US); C12N 2800/30 (2013.01 - EP US)
Citation (search report)
See references of WO 2004048581A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
FR 2847588 A1 20040528; FR 2847588 B1 20060728; AU 2003295051 A1 20040618; CA 2506763 A1 20040610; EP 1563080 A1 20050817; US 2006234966 A1 20061019; WO 2004048581 A1 20040610
DOCDB simple family (application)
FR 0214600 A 20021121; AU 2003295051 A 20031121; CA 2506763 A 20031121; EP 03786045 A 20031121; FR 0303447 W 20031121; US 53569205 A 20050914