EP 1567648 A1 20050831 - METHODS FOR THE PRODUCTION OF THE ANTIBODY MAK F19 COMPRISING THE SELECTION FOR HIGH-PRODUCING RECOMBINANT CELLS
Title (en)
METHODS FOR THE PRODUCTION OF THE ANTIBODY MAK F19 COMPRISING THE SELECTION FOR HIGH-PRODUCING RECOMBINANT CELLS
Title (de)
VERFAHREN ZUR HERSTELLUNG DES ANTIKÖRPERS MAK F19 WELCHES DIE SELEKTION FÜR HOCHPRODUZIERENDE REKOMBINANTE ZELLEN BEINHALTET
Title (fr)
PROCEDES POUR LA PRODUCTION D'ANTICORPS MAK F19 COMPRENANT LA SELECTION DE CELLULES RECOMBINANTES HAUTEMENT PRODUCTIVES
Publication
Application
Priority
- DE 10256083 A 20021129
- EP 0313225 W 20031125
Abstract (en)
[origin: WO2004050879A1] Disclosed is an expression vector for eukaryotic cells comprising a gene that codes for a protein of interest in a functional bond with a hamster ubiquitin/S27a promoter, and a gene which codes for a fluorescent protein. Preferably, said expression vector also comprises an amplifiable selection marker gene. Also disclosed are host cells, preferably mammal cells, which are transfected with the expression vector, methods for producing heterologous gene products, and a method for selecting high-producing cells.
IPC 1-7
IPC 8 full level
C12N 15/65 (2006.01); C12N 15/69 (2006.01)
CPC (source: EP KR)
C12N 15/65 (2013.01 - EP KR); C12N 15/69 (2013.01 - EP); C12N 15/85 (2013.01 - KR)
Citation (search report)
See references of WO 2004050879A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
Designated extension state (EPC)
LT LV
DOCDB simple family (publication)
WO 2004050879 A1 20040617; AR 042244 A1 20050615; AU 2003292102 A1 20040623; BR 0316510 A 20051004; CA 2507714 A1 20040617; CN 1717486 A 20060104; DE 10256083 A1 20040812; EP 1567648 A1 20050831; JP 2006507829 A 20060309; KR 100820035 B1 20080410; KR 20050085203 A 20050829; MX PA05005482 A 20050725; TW 200502388 A 20050116; TW I321152 B 20100301
DOCDB simple family (application)
EP 0313225 W 20031125; AR P030104395 A 20031128; AU 2003292102 A 20031125; BR 0316510 A 20031125; CA 2507714 A 20031125; CN 200380104426 A 20031125; DE 10256083 A 20021129; EP 03767642 A 20031125; JP 2004556182 A 20031125; KR 20057009693 A 20050527; MX PA05005482 A 20031125; TW 92133164 A 20031126