EP 1567656 A2 20050831 - METHOD AND APPARATUS FOR PERFORMING SUBMICROLITER REACTIONS WITH NUCLEIC ACIDS OR PROTEINS
Title (en)
METHOD AND APPARATUS FOR PERFORMING SUBMICROLITER REACTIONS WITH NUCLEIC ACIDS OR PROTEINS
Title (de)
VERFAHREN UND VORRICHTUNG ZUR DURCHFÜHRUNG VON SUBMIKROLITERREAKTIONEN MIT NUKLEINSÄUREN ODER PROTEINEN
Title (fr)
METHODE ET APPAREIL DE MISE EN OEUVRE DE REACTIONS INFERIEURES A UN MICROLITRE A L'AIDE D'ACIDES NUCLEIQUES OU DE PROTEINES
Publication
Application
Priority
- US 0303986 W 20030207
- US 35564802 P 20020208
- US 35566002 P 20020208
- US 26247602 A 20020930
Abstract (en)
[origin: WO03066667A2] Methods for preparing nanoscale reactions using nucleic acids or proteins are presented, Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Proteins are captured specifically and saturably on the modified inner surface of the reaction chamber, typically a capillary. Excess protein is removed and the reaction is performed directly within the capillary. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput reactions involving nucleic acids or proteins are also provided.
IPC 1-7
IPC 8 full level
B01L 3/02 (2006.01); B01L 7/00 (2006.01); C12N 15/09 (2006.01); C12P 1/00 (2006.01); C12Q 1/00 (2006.01); C12Q 1/68 (2006.01); G01N 21/78 (2006.01); B01L 3/00 (2006.01); C40B 40/06 (2006.01); C40B 60/14 (2006.01)
CPC (source: EP)
B01L 3/0241 (2013.01); B01L 7/52 (2013.01); B01L 9/065 (2013.01); B01J 2219/00286 (2013.01); B01J 2219/00369 (2013.01); B01J 2219/00511 (2013.01); B01J 2219/00585 (2013.01); B01J 2219/00691 (2013.01); B01J 2219/00702 (2013.01); B01J 2219/00722 (2013.01); B01L 3/5025 (2013.01); B01L 2300/0838 (2013.01); B01L 2300/1844 (2013.01); B01L 2400/0409 (2013.01); C40B 40/06 (2013.01); C40B 60/14 (2013.01)
Citation (search report)
See references of WO 03066667A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT SE SI SK TR
DOCDB simple family (publication)
WO 03066667 A2 20030814; WO 03066667 A3 20050602; AU 2003217369 A1 20030902; AU 2003217369 A8 20030902; CA 2474429 A1 20030814; EP 1567656 A2 20050831; JP 2005525797 A 20050902
DOCDB simple family (application)
US 0303986 W 20030207; AU 2003217369 A 20030207; CA 2474429 A 20030207; EP 03713412 A 20030207; JP 2003566038 A 20030207