EP 1567664 A2 20050831 - FLUORESCENCE-QUENCHING USED TO DETECT NUCLEIC ACID OLIGOMER HYBRIDIZATION EVENTS AT HIGH SALT CONCENTRATIONS
Title (en)
FLUORESCENCE-QUENCHING USED TO DETECT NUCLEIC ACID OLIGOMER HYBRIDIZATION EVENTS AT HIGH SALT CONCENTRATIONS
Title (de)
FLUORESZENZ-QUENCHEN ZUR DETEKTION VON NUKLEINSÄURE-OLIGOMER-HYBRIDISIERUNGSEREIGNISSEN BEI HOHEN SALZ-KONZENTRATIONEN
Title (fr)
EXTINCTION DE FLUORESCENCE POUR DETECTER DES PHENOMENES D'HYBRIDATION D'OLIGOMERES D'ACIDE NUCLEIQUE EN PRESENCE DE FORTES CONCENTRATIONS DE SEL
Publication
Application
Priority
- DE 0204148 W 20021108
- DE 10155055 A 20011109
Abstract (en)
[origin: WO03040680A2] The invention relates to a method for detecting nucleic acid oligomer hybridization events by fluorescence quenching, which comprises as a first step the provision of a modified surface. The modification of the surface consists in the binding of at least one type of modified nucleic acid oligomers 201, wherein said nucleic acid oligomers 201 are modified by at least one type of fluorophore 102 bound to it. The further steps of the inventive method are: providing a sample that includes nucleic acid oligomers, contacting said sample with the modified surface, adjusting a defined salt concentration of greater than 0,5 mole/l in the solution surrounding the modified nucleic acid oligomer, detecting the fluorescence of the fluorophore and comparing the detected fluorescence intensity with reference values.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01); C12Q 1/6818 (2018.01)
CPC (source: EP US)
C12Q 1/6818 (2013.01 - EP US)
C-Set (source: EP US)
Citation (search report)
See references of WO 03040680A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR
DOCDB simple family (publication)
WO 03040680 A2 20030515; WO 03040680 A3 20040129; AU 2002363453 A1 20030519; DE 10155055 A1 20030528; EP 1567664 A2 20050831; US 2006188877 A1 20060824
DOCDB simple family (application)
DE 0204148 W 20021108; AU 2002363453 A 20021108; DE 10155055 A 20011109; EP 02802612 A 20021108; US 52947202 A 20021108