EP 1578951 A4 20051116 - DNA POLYMERASES WITH REDUCED BASE ANALOG DETECTION ACTIVITY
Title (en)
DNA POLYMERASES WITH REDUCED BASE ANALOG DETECTION ACTIVITY
Title (de)
DNA-POLYMERASE MIT REDUZIERTER NACHWEISAKTIVITÄTFÜR BASENANALOGE
Title (fr)
ADN POLYMERASES A ACTIVITE DE DETECTION ANALOGIQUE A BASE REDUITE
Publication
Application
Priority
- US 0333997 W 20031024
- US 28096202 A 20021025
- US 29868002 A 20021118
- US 40860103 A 20030407
Abstract (en)
[origin: WO2004038007A2] The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal DNA polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3'-5' exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.
IPC 1-7
IPC 8 full level
C07H 21/04 (2006.01); C12N 1/21 (2006.01); C12N 9/12 (2006.01); C12N 9/22 (2006.01); C12N 15/03 (2006.01); C12P 19/34 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C07H 21/04 (2013.01); C12N 9/1252 (2013.01); C12Q 1/6869 (2013.01)
Citation (search report)
- [E] WO 03089637 A1 20031030 - UNIV NEWCASTLE [GB], et al
- [A] WO 0192501 A1 20011206 - MJ BIOWORKS INC [US], et al
- [PA] WO 03060144 A2 20030724 - STRATAGENE INC [US], et al
- [A] GARDNER ANDREW F ET AL: "Determinants of nucleotide sugar recognition in an archaeon DNA polymerase", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 12, 15 June 1999 (1999-06-15), pages 2545 - 2553, XP002163101, ISSN: 0305-1048
- [PX] FOGG MARK J ET AL: "Structural basis for uracil recognition by archaeal family B DNA polymerases.", NATURE STRUCTURAL BIOLOGY. UNITED STATES DEC 2002, vol. 9, no. 12, December 2002 (2002-12-01), pages 922 - 927, XP002250141, ISSN: 1072-8368
- [PA] CONNOLLY B A ET AL: "Uracil recognition by archaeal family B DNA polymerases.", BIOCHEMICAL SOCIETY TRANSACTIONS. ENGLAND JUN 2003, vol. 31, no. Pt 3, June 2003 (2003-06-01), pages 699 - 702, XP009015138, ISSN: 0300-5127
- See references of WO 2004038007A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2004038007 A2 20040506; WO 2004038007 A3 20050210; AU 2003301590 A1 20040513; CA 2503038 A1 20040506; EP 1578951 A2 20050928; EP 1578951 A4 20051116; JP 2006507012 A 20060302
DOCDB simple family (application)
US 0333997 W 20031024; AU 2003301590 A 20031024; CA 2503038 A 20031024; EP 03809647 A 20031024; JP 2005501697 A 20031024