Global Patent Index - EP 1581630 A4

EP 1581630 A4 20061011 - DNA POLYMERASE BLENDS AND USES THEREOF

Title (en)

DNA POLYMERASE BLENDS AND USES THEREOF

Title (de)

DNA-POLYMERASE-MISCHUNGEN UND IHRE VERWENDUNG

Title (fr)

MELANGES D'ADN-POLYMERASE ET LEURS UTILISATIONS

Publication

EP 1581630 A4 20061011 (EN)

Application

EP 03797055 A 20031218

Priority

  • US 0340421 W 20031218
  • US 32484602 A 20021220
  • US 70240003 A 20031105

Abstract (en)

[origin: WO2004058942A2] The present invention discloses novel blends of chimeric and non-chimeric thermostable DNA polymerases for use in PCR, DNA sequencing and mutagenesis protocols. The invention allows for PCR reactions with shorter extension times that will facilitate PCR amplification of genomic DNA templates and improve the efficacity of long PCR.

IPC 1-7

C12N 9/12

IPC 8 full level

C12N 9/12 (2006.01)

CPC (source: EP US)

C12N 9/1241 (2013.01 - EP US); C12N 9/1252 (2013.01 - EP US)

Citation (search report)

  • [AD] WO 0192501 A1 20011206 - MJ BIOWORKS INC [US], et al
  • [AD] US 5489523 A 19960206 - MATHUR ERIC J [US]
  • [AD] PAVLOV A R ET AL: "Helix-hairpin-helix motifs confer salt resistance and processivity on chimeric DNA polymerases", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 99, no. 21, 15 October 2002 (2002-10-15), pages 13510 - 13515, XP002345273, ISSN: 0027-8424
  • [AD] BARNES W M: "PCR AMPLIFICATION OF UP TO 35-KB DNA WITH HIGH FIDELITY AND HIGH YIELD FROM LAMBDA BACTERIOPHAGE TEMPLATES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 91, March 1994 (1994-03-01), pages 2216 - 2220, XP002030133, ISSN: 0027-8424
  • [A] SHIMAZAKI NORIKO ET AL: "Over-expression of human DNA polymerase lambda in E. coli and characterization of the recombinant enzyme", GENES TO CELLS, vol. 7, no. 7, July 2002 (2002-07-01), pages 639 - 651, XP002382561, ISSN: 1356-9597
  • See references of WO 2004058942A2

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

DOCDB simple family (publication)

WO 2004058942 A2 20040715; WO 2004058942 A3 20051229; AU 2003297982 A1 20040722; CA 2511227 A1 20040715; EP 1581630 A2 20051005; EP 1581630 A4 20061011; EP 1581630 B1 20120627; JP 2006513726 A 20060427; US 2004197800 A1 20041007; US 7960157 B2 20110614

DOCDB simple family (application)

US 0340421 W 20031218; AU 2003297982 A 20031218; CA 2511227 A 20031218; EP 03797055 A 20031218; JP 2005510011 A 20031218; US 70240003 A 20031105